|Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Therapeutic modalities for male infertile patients have never been established because of various causes of this disease. In this study, we tried to clarify the causes of male infertility by clinical, genetic and epidemiological approaches.
When normal findings of sperm counts and sperm motility were obtained in seminalysis, patients were diagnosed normal. However, it has been suspected that some of them might have functional abnormalities of their sperm including acrosomal reaction, if their partners did not show any problem in gynecological examinations. Quality of sperm motility was testing by CASA (Computed Assisted Sperm Analyzer) in male infertility with normal sperm counts and sperm motility and fertile male volunteers. Unfortunately, no significant difference in sperm motile function was recognized between two groups. As well as CASA, using PENETRAK and acrobeads test, no significant difference in sperm function was shown between infertile and fertile men.
On the other hand, to s
tudy the causes of hypospermatogenesis in patients with infertility, potentiality of apoptosis and cell proliferation was immunohistochemically evaluated using TUNEL method and the detection of the expression of BrdU, respectively. Based on these results, the causes of hypospermatogenesis was not uniform, some of them showed the decrease of spermatogonial renewal due to the increase of its apoptosis.
As an epidemiological study, sperm count and sperm motility were examined in normal young healthy males in the Sapporo area. These values were not significantly changed compared to those of the past 20 years. There was no significant difference between men who have a child and a whole group. It was not obvious that male infertility was increasing.
From these results, it was concluded that issues of hypospermatogenesis would be more crucial than sperm function as a cause of male infertility. Recently, deterioration of sperm function was suggested, however, no evidence of the decrease of sperm counts was epidemiologically detected. Less