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Studies on a KRAB zinc finger gene, highly expressed in the human testis

Research Project

Project/Area Number 10671487
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionYokohama City University

Principal Investigator

TAKEDA Mitsumasa  Yokohama City Univ., Urology, assistant professor, 医学部・附属病院, 講師 (70244499)

Co-Investigator(Kenkyū-buntansha) HOSAKA Masahiko  Yokohama City Univ., Urology, professor, 医学部, 教授 (30106330)
NOGUCHI Kazumi  Yokohama City Univ., Urology, associate, 医学部・附属病院, 助教授 (10164675)
SAITO Kazuo  Yokohama City Univ., Urology, assistant, 医学部・附属病院, 講師 (90264647)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordszinc finger gene / germ cell / spermatogenesis / male infertility / testicular tumour / 特発性不妊症
Research Abstract

Six Kruppe1-type zinc finger (ZF) genes were cloned from a seminoma cDNAlibrary. One, ZFS-1, showed highly sequence homology to the ZNF91 KRAB (Kruppe1-associated box) ZF gene family and also the same chromosomal assignment. Interestingly, Nortern blot analysis revealed that multiple ZF genes on chromosome 19 were predominantly expressed in seminoma and adult normal human testis, In addition, the testis and the seminoma showed a unique expression of 2.3 kb transcript that was thought to be an existence of germ cell specific zinc finger gene. Then, using ZFS-1 as a probe TSF (Testis specific zinc finger gene) was cloned from a human adult testis library. Sequence analysis demonstrated this 2371 base-paired gene codes 597 amino acids and have a KRAB domain, a Zinc finger domain in the N-terminal and C-terminal, respectively.
Our results suggest that TSF gene is a member of the ZNF91 family and may be imlicated in the development and/or growth of germ cell and seminoma.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] T.Ogawa: "Enhaunced Expression in Seminoma of human Zinc Finger Genes Located on Chromosome 19"Cancer Genet Cytogenet. 97. 1-7 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Kanno: "Voiability and function of human sperm in electrolyte-free cold preservation"Fertil Steril. 69. 127-131 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Noguchi: "Hypogonadotropic Hypogonadism-Piagnosis and Treatment of 30 cases"Jpn J Fertil Steril. 44. 173-178 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 田中克幸: "Differential Display法を用いたラット前立腺におけるアンドロゲン応答遺伝子の検索"ホルモンと臨床-ステロイドホルモン研究の進歩1999. 47. 155-160 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 武田光正: "テストステロン合成阻害剤-新しい治療法・新薬開発動向"日本臨床. 58. 312-316 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Ogawa: "Enhaunced Expression in Seminoma of Human Zinc Finger Genes Located on Chromosome 19"Cancer Genet Cytogenet. 100. 36-42 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Kanno: "Viability and functin of human sperm in electrolyte-free cold preservation"Fertil.Steril. 69. 127-131 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Noguchi: "Hypogonadotropic Hypogonadism-Diagnosis and treatment of 30 cases"Jpn J Fertil Steril. 44. 173-178 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Ogawa: "Enhaunced Expression in Seminoms of human Zine Finger Genes Located on Chromosome19"Cancer Genet Cytogenet 97:1-7. 97. 1-7 (1977)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田中 克幸: "Differential display法を用いたラット前立腺におけるアンドロゲン応答遺伝子の検索"ホルモンと臨床,特集99ステロイドホルモン研究. 155-160 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Poncelet DA: "Functional analysis of ZNF85KRAB Zinc finger protein,a member of the highly homologous ZNF91 family"DNA Cell Biol. 17. 931-943 (1998)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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