| Project/Area Number |
10671492
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Keio University |
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Principal Investigator |
SUZUKI Yuriko KEIO UNIV. SCH. MED., INSTRUCTOR, 医学部, 助手 (40255435)
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| Co-Investigator(Kenkyū-buntansha) |
MURAI Masaru KEIO UNIV. SCH. MED., PROFESSOR, 医学部, 教授 (90101956)
IKEDA Hideyuki KEIO UNIV. SCH. MED., ASSISTANT PROFESSOR, 医学部, 講師 (40301494)
KAWAKAMI Yutaka KEIO UNIV. SCH. MED., PROFESSOR, 医学部, 教授 (50161287)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | ANTIGEN OF RCC / SEREX |
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| Research Abstract |
New antigens of renal cell carcinoma were tried to be identified by the method of SEREX (serological analysis of recombinant cDNA expression libraries). Four cell lines of human renal cell carcinoma were used to make cDNA library constructed by lambda ZAP express vector. A library containing 1-2x10ィイD16ィエD1 primary recombinants were obtained. The SEREX method of immnoscreening cDNA expression library with 4 allogeneic sera was applied and about 1x10ィイD16ィエD1 recombinants were screened per serum. The expressed proteins induced by IPTG were transferred to nitrocellulose membrane, incubated in a 1:100 dilution of pre-absorbed allogeneic sera, then incubated in a 1:4000 dilution of alkaline phosphatase conjugated Fc γ fragment specific goat anti-human IgG, and processed for color development. We obtained 187 serum reactive clones representing 8 different antigen candidates. Five genes of eight were essential genes and house keeping genes. One candidate is a new gene. Other candidate is KIAA1102. The KIAA putative product is unknown, but have homologous reagion to F box and LIM domain. Another candidate that consists of 176 clones is PARG1 which encodes GTPase-activating protein for Rho interacts with a PDZ domain of the protein-tyrosine phosphatase PTPL1. The product of KIAA1102 and PARG1 did not react with sera from normal donors and colon cancer patient, and the product of PARG1 reacted with five out of ten sera from renal cell carcinoma. These results suggest they are new antigen of renal cell carcinoma.
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