| Project/Area Number |
10671496
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
FURUTA Nozomu (2000) Jikei University School of Medicine. Department of Urology. Lecturer., 医学部, 講師 (70190155)
仲田 浄治郎 (1998-1999) 東京慈恵会医科大学, 医学部, 講師 (60130184)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Yutaka Jikei University School of Medicine. Department of Pathology. Associate Professor., 医学部, 助教授 (60057073)
MATSUURA Tomokazu Jikei University School of Medicine. Department of Internal Medicine. Lecturer., 医学部, 助手 (30199749)
古田 希 東京慈恵会医科大学, 医学部, 講師 (70190155)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Human / Pheochromocytoma / Cultured cell / Morphology / Function / Catecholamine / Adrenocortical steroid / Nerve growth factor |
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| Research Abstract |
Primary cell cultures of three human pheochromocytomas (PC) that were associated with high serum levels of adrenaline and noradrenaline were developed to study the effects of nerve growth factor (NGF) and dexamethasone on the morphology and function of PC cells in vitro.
By phase-contrast microscopy, cultured cells were small and hyperchromatic on the first day of culture, neurite-like processes that extended to other cells developed several days later and were maintained for more than 3 months. NGF (100ng/ml), dexamethasone (10^<-5>M), or NGF + dexamethasone were added to the culture media 2 weeks after the cultured cells had stabilized. Catecholemine concentrations in the medium were maintained at higher levels after addition of NGF, dexamethasone, or NGF + dexamethasone-as compared to control cells. In the presence of NGF, extension of neurite-like processes was clearly accelerated, while high levels of dexamethasone inhibited growth of processes.
These in vitro studies showed that the addition of NGF or the removal of dexamethasone induces differentiation of adrenal neurons present in pheochromocytomas, suggesting that adrenocorical steroid hormones influence the morphological control of adrenal medullary cells.
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