| Project/Area Number |
10671519
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
|
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| Research Institution | University of Tokyo |
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Principal Investigator |
OSUGA Yutoka Faculty of Medicine OB/GYN Associate Professor, 医学部・附属病院, 助手 (80260496)
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| Co-Investigator(Kenkyū-buntansha) |
OSAMU Tsutsumi Faculty of Medicine OB/GYN Associate Professor, 医学部・附属病院分院, 教授 (60134574)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | sex differentiation / Duarian function / SF-1 / DAX-1 / HFG / anglogenin |
|---|
| Research Abstract |
Angiogenesis is an essential event during the development of the ovarian follicle and ensuing formation of the corpus luteum. We investigated the presence of angiogenin, a potent inducer of angiogenesis, and the regulatory mechanisms of its production in the human ovary. Follicular fluid (FF) and granulosa cells (GC) were collected from women undergoing in-vitro fertilization and embryo-transfer. The presence of angiogenin in FF and GC was demonstrated by Western blot analysis. The production of angiogenin by cultured GC was stimulated with the addition of human chorionic gonadotropin (hCG) or cAMP or under the hypoxic milieu. Concentrations of angiogenin in FF from individual follicle were positively correlated with those of progesterone, but not estradiol and testosterone. Given the presence of angiogenin in FF and upregulation of its production by hCG and hypoxia, it seems logical to assume that angiogenin may play a role as a local angiogenic factor in the human ovary.
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