• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Physiological Role of Theca-Granulosa Cell Interactions in Controlling Ovarian Follicular Development and Atresia

Research Project

Project/Area Number 10671527
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionFUKUI MEDICAL UNIVERSITY, DEPARTMENT OF OBSTETRICS AND GYNECOLOGY

Principal Investigator

KOTSUJI Fumikazu  Fukui Medical University, Dept. of OB/GYN, Professor, 医学部, 教授 (50153573)

Co-Investigator(Kenkyū-buntansha) 矢田 敬二  福井医科大学, 医学部, 助手 (00239782)
HOSOKAWA Kumiko  Fukui Medical University, Dept. of OB/GYN, Assistant Professor, 医学部, 講師 (60199495)
GOTO Kenji  Fukui Medical University, Dept. of OB/GYN, Assistant Professor, 医学部・附属病院, 講師 (10178444)
TAJIMA Kimihisa  Fukui Medical University, Dept. of OB/GYN, Instructor, 医学部・附属病院, 助手 (60303377)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsGRANULOSA CELL / THECA CELL / CELLULAR INTERACTION / FOLLICULAR DEVELOPMENT / FOLLICULAR ATRESIA / CELLAR GROWT / ESTRADIOL / PROGESTERONE / APOPTOSIS
Research Abstract

1. We have investigated the possible role of theca and granulosa cell interaction in the control of follicular maturation and atresia during bovine ovarian follicular developmentusng a co-culture system in which granulosa and theca cells were grown on oppositesides of a collagen membrane. We have already demonstrated the following facs.
(1) Granulosa-theca cell interaction is a major follicular constituent in the control of follicular cell differentiation; theca cells secrete factor(s) inhibiting the differentiation of the immature granulosa cells while promoting that of the matured ones and that granulosa cells secrete factor(s) promoting both the differentiation and growth of thecacells throughout the follicular maturation process.
(2) Thecal factor(s) control the apoptosis of granulosa cells; inhibit the apoptosis of granulosa cells at the early and late stage of follicular development. Susceptibility of granulosa cells to apoptosis decreases as follicles grow.
2. In the second series of the study, we established human granulosa cell lines by triply transfected with SV40 DNA, Ha-ras oncogene and the temperature sensitive mutant of p53(p53-val135). Using this cell-line (HO-23), it was confirmed that glucocorticoids and extracellular matrix(ECM) enhanced P4 production while bFGF and phorbol ester TPA suppressed it, that glucocorticoids, ECM and bFGF inhibited p53-induced apoptosis of HO-23 cells while TPA enhanced it, and that apoptosis at early stage does not interrupt steroidogenesis.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 細川久美子ら: "ヒト顆粒膜細胞株の樹立とこれを用いたホルモン産生アポトーシス発現機序の研究"産婦人科治療. 78. 1172 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Yada et al: "The role of ovarian theca and granulosa cell interaction in hormone production and cell growth during the bovine follicular maturation process"Biol Reprod. 61. 1480-1486 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A Amsterdam et al: "Structure-function rekationship in the normal and malignant ovary during programmed cell death"Acta Histochem Cytochem. 32. 127-131 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A Amsterdam et al: "Crosstalk among multiple signaling pathways controlling ovarian cell death"Trend in Endocrinology and Metabolism. 10. 255-261 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Sasaki et al: "Apoptosisi and Chemoresisitance in human ovarian cancer"Biological Signals and Receptors.. ( in press ).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Sasaki et al: "Frontiers in Endocrinology Ovarian Function Research : Present and Future"Serono Symposia Puburications. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Yada et al: "The role ovarian theca and granulosa cell interaction in hormone production and cell growth during the bovine follicular maturation process."Biol Reprod. 61. 1480-1486 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A Amsterdam et al: "Structure-function relationship in the normal and malignant ovary during programmed cell death"Acta Histochem Cytochem. 32(2). 127-131 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A Amsterdam et al: "Crosstalk among multiple signaling pathways controlling ovarian cell death"Trend in Endocrinology and Metabolism. 10(7). 255-261 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Sasaki et al: "Apoptosis and Chemoresistance in human ovarian cancer"Biological Signals and Receptors. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Sasaki et al: "Frontiers in Endocrinology Ovarian Function Research : Present and Future"Serono Symposia Puburications. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 細川久美子ら: "ヒト顆粒膜細胞株の樹立とこれを用いたホルモン産生アポトーシス発現機序の研究"産婦人科治療. 78・6. 1172 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirotsugu Yada etc.: "The role of ovarian theca and granulosa cell interaction in hormone production and cell growth during the bovine follicular maturation process."Biol Reprod. 61. 1480-1486 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] A Amsterdam etc.: "Structure-function relationship in the normal and malignant ovary during programmed cell death."Acta Histochem Cytochem. 32・2. 127-131 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Amsterdam A etc.: "Crosstalk among multiple signaling pathways controlling ovarian cell death."Trend in Endocrinology and Metabolism. 10・7. 255-261 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sasaki H etc.: "Apoptosis and Chemoresistance in human ovarian cancer."Biological Signals and Receptors.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Sasaki H etc.: "Frontiers in Endocrinology Ovarian Function Research : Present and Future"Serono Symposia Puburications. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 細川久美子等: "Induction of Ad4BP/SF-1, steroidogenic acute regulatory protein, and cytochrome P450scc enzyme system expression in newly established human granulosa cell lines." Endocrinology. 139巻. 4679-4687 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 細川久美子等: "Modulation of Mdm2 expression and p53-induced apoptosis in immortalized human ovarian granulosa cells." Endocrinology. 139巻. 4688-4700 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 細川久美子等: "ヒト顆粒膜細胞株の樹立とこれを用いたホルモン産生、アポトーシス機構の研究" 産婦人科治療. in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] 小辻文和: "排卵と月経:顆粒膜-莢膜細胞間相互作用による卵胞機能調節" 中山書店, 193-205 (1998)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi