| Project/Area Number |
10671532
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Mie University |
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Principal Investigator |
TOYODA Nagayasu Mie University, Faculty of Medicine, Professor, 医学部, 教授 (40126983)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Masazumi Mie University, Hospital, Lecturer, 医学部・附属病院, 講師 (00159914)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | pregnancy / adipose tissue / rats / leptin / TNF-α / PPAR-γ / mRNA / GLUT4 / 産褥 |
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| Research Abstract |
Serum leptin levels in pregnant rats were stable except on day 20 of pregnancy and significantly reduced during lactation compared to nonpregnant rats. Leptin mRNA in parametrial adipose tissue reflected the circulating levels, also being significantly reduced during late pregnancy and lactation. Leptin mRNA expression was observed in placenta, but the amount suggested little influence on circulating leptin levels. This might be a reason why there was a difference in leptin levels during pregnancy between human and rats. These results indicate that reduction in leptin mRNA in parametrial adipose tissue and circulating leptin levels may increase appetite during late pregnancy and lactation and may play a role in regulating metabolic homeostasis around parturition in rats.
Serum TNF-α levels and mRNA abundance in adipose tissue during pregnancy and lactation did not change significantly. In addition, the abundance of PPAR-γ mRNA in parametrial adipose tissue during pregnancy and lactation did not change at all. To elucidate the mechanism of a marked decrease in adipose tissue volume after delivery, the existence of apoptosis in perinatal period has been investigated now.
The expression of glucose transporter 4 (GLUT4) protein was assessed by Western blotting in streptozotocin-induced diabetic pregnant rats. In adipose tissue, relative to the normal nonpregnant rats, it was significantly decreased by 30% in the normal pregnant rats, by 37% in the diabetic nonpregnant rats. Furthermore, the amount of GLUT4 in the diabetic pregnant rats was markedly reduced by 35%. Now, we have assessed the expression of adipocytokines in diabetic pregnant rats. Then, it was suggested that sex steroid hormones might be one of the factors that regulate GLUT4 mRNA expression in adipose tissue.
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