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Study for tumor cell-stromal cell interaction related with Platelet-derived growth factor produced by ovarian cancer

Research Project

Project/Area Number 10671552
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKyushu University

Principal Investigator

KOBAYASHI Hiroaki  Kyushu Univ., Faculty of Medicine, Lecturer, 医学部, 助手 (70260700)

Co-Investigator(Kenkyū-buntansha) KAKU Tunehisa  Kyushu Univ., School of Health Science, Associate Professor, 医療技術短期大学部, 助教授 (60185717)
HIRAKAWA Toshio  Kyushu Univ., Faculty of Medicine, Senior Lecturer, 医学部, 講師 (20218770)
KATO Kiyoko  Kyushu Univ., Medical Institute of Bioregulation, Senior Lecturer, 生体防衛医学研究所, 講師 (10253527)
KAMURA Toshiharu  Kyushu Univ., Faculty of Medicine, Associate Professor, 医学部, 助教授 (30152870)
WAKE Norio  Kyushu Univ., Medical Institute of Bioregulation, Professor, 生体防衛医学研究所, 教授 (50158606)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsOvarian cancer / calponin / PDGF / Cell-cell interaction / Cancer invasion / 平滑筋型αアクチン / 間質細胞 / 細胞骨格蛋白 / 肝細胞増殖因子 / 癌細胞の浸潤
Research Abstract

Platelet-derived growth factor (PDGF), sis-oncogene product, was rarely detected in the fluid of benign ovarian tumors. On the other hand the fluid of borderline malignant ovarian tumors often contained low levels of PDGF and the fluid of most ovarian cancers contained PDGF at much higher levels. A Northern blot analysis revealed that the expression of calponin, one of actin-binding cytoskeletal proteins, in fibroblasts or peritoneal methothelial cells was inhibited by the PDGF-containing conditioned medium (CM) of human ovarian cancer cell lines. We confirmed that human recombinant PDGF also decreased the calponin expression in them in a dose-dependent fashion. Our collaborators, Dr. Shu'nichiro Taniguchi et. al. (Faculty of Medicine, Shinshu University), reported that the calponin gene-knockout mouse showed an enhanced peritoneal dissemination of ovarian cancer, which suggested that the decreased expression of calponin in the host peritoneal methothelial cells was related with the increased invasiveness of ovarian cancer cells.
Therefore, it is possible that an ovarian cancer itself induces an advantage for their invasion and metastasis through tumor cell-stromal cell interaction which producing impairment of host stromal defense mechanism. This hypothesis might be helpful to develop a new treatment strategy against the peritoneal dissemination of human ovarian cancer.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Kaku T, Kobayashi H, et al: "Angiogenesis in adenocarcinoma of the uterine cervix."83. 1384-1390 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Vogt V, Kobayashi H, et al.: "Overexpression of Lerk-5/Eplg5 messenger RNA: A novel marker for increased tumorigenicity and metastatic potential in human malignant melanomas."Clinical Cancer Research. 4. 791-797 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mitsumoto M, Kobayashi H, et al.: "Emergence of higher levels of invasive and metastaic properties in the drug resistant cancer cell lines after the repeated administration of cisplatin in tumor-bearing mice."Journal of Cancer Research and Clincial Oncology. 124. 607-614 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kaku T, Kobayashi H, et al.: "Endometrial carcinoma associated with hyperplasia-Immunohistochemical study of angiogenesis and p53 expression"Gynecologic Oncology. 72. 51-55 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kamura T, Kobayashi H, et al: "Comparsion of p53 and CD44 variant6 expression between paired primary and recurrent ovarian cancer-An Immunohistochemical Analysis."Oncology Report. 6. 97-101 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Kaku et. al.: "Angiogenesis in Adenocarcinoma of the Uterine Cervix."Cancer. 83(7). 1384-1390 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Vogt et. al.: "Overexpression of Lerk-5/Eplg5 Messenger RNA : A Novel Marker for Increased Tumorigenicity and Metastatic Potential in Human Malignant Melanomas"Clinical Cancer Research. 4. 791-797 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Mitsumoto et. al.: "Emergence of higher levels of invasive and metastatic properties in the drug resistant cancer cell lines after the repeated administration of cisplatin in tumor-bearing mice"J Cancer Res Clin Oncol. 124. 607-614 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Kaku et. al.: "Endometrial Carcinoma Associated with Hyperplasia - Immunohistochemical Study of Angiogenesis and p53 Expression"Gynecologic Oncology. 72. 51-55 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Kamura et. al.: "Comparison of p53 and CD44 variant 6 expression between paired primary and recurrent ovarian cancer : An immunohistochemical analysis"Oncology Reports. 6. 97-101 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kaku T,Kobayashi H, et al.: "Angiogenesis in adenocarcinoma of the uterine cervix"Cancer. 83. 1384-1390 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Vogt V,Kobayashi H, et al.: "Overexpression of Lerk-5/Eplg5 messenger RNA : A novel marker for increased tumorigenicity and metastatic potential in human malignant melanomas"Clinical Cancer Research. 4. 791-797 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mitsumoto M,Kobayashi H, et al.: "Emergence of higher levels of invasive and metastatic properties in the drug resistant cancer cell lines after the repeated administration of cisplatin in tumor-bearing mice"Journal of Cancer Research and Clinical Oncology. 124. 607 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kaku T,Kobayashi H, et al.: "Endometrial carcinoma associated with hyperplasia-Immunohistochemical study of angiogenesis and p53 expression"Gynecologic Oncology. 72. 51-55 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kamura T,Kobayashi H, et al.: "Comparison of p53 and CD44 variant6 expression between paired primary and recurrent ovarian cancer-An Immunohistochemical Analysis"Oncology Report. 6. 97-101 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kaku T,Kobayashi H, et al.: "Angiogenesis in adenocarcinoma of the uterine cervix." Cancer. 83. 1384-1390 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Vogt V,Kobayashi H, et al.: "Overexpression of Lerk-5/Eplg5 messenger RNA: A novel marker for increased tumorigenicity and metastatic potential in human malignant melanomas." Clinical Cancer Research. 4. 791-797 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mitsumoto M,Kobayashi H, et al.: "Emergence of higher levels of invasive and metastatic properties in the drug resistant cancer cell lines after the repeated administration of cisplatin in tumor-bearing mice." Journal of Cancer Research and Clinical Oncology. 124. 607-614 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kaku T,Kobayashi H, et al.: "Endometrial carcinoma associated with hyperplasia-Immunohistochemical study of angiogenesis and p53 expression" Gynecologic Oncology. 72. 51-55 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kamura T,Kobayashi H, et al.: "Comparison of p53 and CD44 variant6 expression between paired primary and recurrent ovarian cancer-An Immunohistochemical Analysis." Oncology Report. 6. 97-101 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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