| Project/Area Number |
10671557
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | NAGASAKI UNIVERSITY |
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Principal Investigator |
FUJISHITA Akira Department of Obstetrics Gynecology, Nagasaki University School of Medicine, Assist Professor, 医学部・附属病院, 講師 (70190030)
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| Co-Investigator(Kenkyū-buntansha) |
KOJI Takehiko Nagasaki University School of Medicine, Professor, 医学部, 教授 (30170179)
ISHIMARU Tadayuki Department of Obstetrics Gynecology, Nagasaki University School of Medicine, Professor, 医学部, 教授 (20039580)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | endometriosis / immunohistochemical study / growth factors / angiogenic factor / peritoneal fluid / macrophage / cytokines / infertility |
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| Research Abstract |
We studied the pathogenesis of pelvic endometriosis using experimental animal model and morphological examination of many samples of pelvic biopsy. We also investigated the influence of pelvic endometriosis and ovarian endometrioma on the outcome of fertility.
Our study demonstrated that the expressions of growth factors such as PCNA, HGF, c-Met and VEGF were markedly higher in red pigmented lesions compared with black or white lesions of pelvic peritoneum. The ahgiogenic markers such as VEGF or endoglin had also similar high activity in red lesions compared to other pigmented lesions. We also found that active endometriotic lesions and their I adjacent peritoneum accompanied by an increased infiltration of macrophages could be responsible for the reactive change of mesothelial cells in pelvic peritoneum. These results suggest that the pathogenesis and activity of endometriosis possibly depend on internal angiogeneisis being triggered by macrophages.
There was no significant difference in pregnancy rate among r-AFS stages. However our TOP classification was useful to predict the future pregnancy. The most important factor was tubal condition such as tubal adhesions and occlusion and this was not affected by the size and location of ovarian endometrioma. We need to establish a new classification that predicts not only the degree of symptoms, but also one that helps to assess the future reproductive outcome of women with endometriosis.
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