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Analysis of glycosyltransferase involved in metastasis of uterine endometrial cancer

Research Project

Project/Area Number 10671569
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKeio University

Principal Investigator

KUBUSHIRO Kaneyuki  Keio Univ.of Med.Assistant Professor, 医学部, 講師 (50170022)

Co-Investigator(Kenkyū-buntansha) TSUKAZAKI Katsumi  Keio Univ.of Med.Assistant Professor, 医学部, 助教授 (40118972)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Keywordsuterine endometrial cancer / adhesion / β1,4 galactosyltransferase / northern blot analysis / laminin / collagen type IV / immunohistochemical staining / 遺伝子導入 / 転移 / β1-4ガラクトース転移酵素 / 転移能
Research Abstract

Expression of β1,4 galactosyltransferase (β1,4GT) gene products in eleven gynecological cancer cell lines was examined. A 4.7 kb mRNA and protein (54,000 Da and 57,000 Da) were detected by Northern blot and western blot analysis. Immunohistochemical staining revealed that β1,4GT was localized in the Golgi or ER of tumor cells. An intense β1,4GT mRNA signal was detected in ovarian and uterine cervical cancer cells, whereas the level of β1,4GT mRNA was very low in uterine endometrial cancer cells. We confirmed that expression of β1,4GT mRNA corresponded to expression of β1,4GT protein.These results suggested that expression of the β1,4GT gene products is higher in cervical and ovarian cancer cells than those in endometrial cancer cells. Then we transfected human β1,4GT cDNA into uterine endometrial cancer cell line, SNG- M.Northern blot analysis was used to select 5 stable transfectants. β1,4GT mRNA of 5 trasfectants were overexpressed by approximately 6-fold compared to vector control cells ancd parental SNG-M cells. Further we analyzed the biological effects of overexpression of β1,4GT in endometrial cancer cells. In vitro study has showed a significant increase of cell growth and increase of adhesion activity to laminin and collagen type IV compared to vector control cells. These data indicate that overexpression of β1,4GT gene products may change the biological properties of uterine endometrial cancer cells.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Yoshibumi Kaneta,Kaneyuki Kubushiro, et al.: "Effect of gelonin immunoconjugate with monoclonal antibody MSN-1 to endometrial adenocarcinoma on antigen-producing tumor cells in vitro."Japanese Journal of Cancer Research. 89. 583-588 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsumi Tsukazaki,Kaneyuki Kubushiro, et al.: "Glycoconjugates in endometrial cancer."Acta Cytologoca. 42. 587-588 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koji Kobiki,Kaneyuki Ktubushiro, et al.: "Detection ofendometrial cancer by flow cytometry using two monoclonal antibodies."Cytometry. 36. 150-156 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takuya Irie,Kaneytuki Kubushiro, et al.: "Inhibition of attachment and chemotactic invasion of uterine endometrial cancer cells by a new vinca alkaloid, conophylline."Anticancer Research. 19. 3061-3066 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro, et al.: "Changes of β-1,4-galactosyltransferase with the development of endometrial cancer."Gynecologic and Obstetric Investigation. 48. 211-214 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kaneytuki Kubushiro, et al.: "Biological characteristics of human uterine endometrial cancer variant cells selicted for blood group H type 1 antigen : adhesion to vascular endothelial cells."Acta Histochemica et Cytochemica. 33(3). 209-213 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshibumi Kaneta, Kaneyuki Kubushiro, et al.: "Effect of gelonin immunoconjugate with monoclonal antibody MSN-1 to endometrial adenocarcinoma on antigen-producing tumor cells in vitro."Japanese Journal of Cancer Research. 89. 583-588 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsumi Tsukazaki, Kaneyuki Kubushiro, et al.: "Glycoconjugates in endometrial cancer."Acta Cytologica. 42. 587-588 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koji Kobiki, Kaneyuki Kubushiro, et al.: "Detection of endometrial cancer by flow cytometry using two monoclonal antibodies"Cytometry. 36. 150-156 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takuya Irie, Kaneyuki Kubushiro, et al.: "Inhibition attachment and chemotactic invasion of uterine endometrial cancer cells by a new vinca alkaloid, conophylline."Anticancer Research. 19. 3061-3066 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro, et al.: "Changes of β-1,4-galactosyltransferase with the development of endometrial cancer."Gynecologic and Obstetric Investigation. 48. 211-214 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro, et al.: "Biological characteristics of human uterine endometrial cancer variant cells selected for blood group H Type 1 antigen : adhesion to vascular endothelial cells."Acta Histochemica et Cytochemica. 33(3). 209-213 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshibumi Kaneta, Kaneyuki Kubushiro, et al.: "Selective cytotoxicity of adriamycin immunoconjugate of monoclonal antibody MSN-1 to endometrial adenocarcinoma in vitro and in vivo."Oncology Reports. 7. 1099-1106 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro, et al.: "Biological characteristics ofhuman uterine endometrial cancer variant cells selected for blood group H type 1 antigen : adhesion to vascular endothelial cells."Acta Histochemica et Cytochemica. 33・3. 744-747 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshibumi Kaneta,Kaneyuki Kubushiro, et al.: "Selective cytotoxicity ofadriamycin immunoconjugate of monoclonal antibody MSN-1 to endometrial adenocarcinoma in vitro and in vivo."Oncology Reports. 7. 1099-1106 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Koji Kobiki,Kaneyuki Kubushiro,et.al.: "Detection if endometrial cancer by flow cytometry using two monoclonal antibodies"Cytometry. 36. 150-156 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takuya Irie,Kaneyuki Kubushiro,et.al.: "Inhibition Attachment and Chemotactic Invasion of Uterine Endometrial Cancer Cells by a New Vinca Alkaloid,Conophylline"Anticancer Research. 19. 3061-3066 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 久布白兼行 他: "妊娠時の子宮頸部初期癌と円錐切除術"産婦人科治療. 78(6). 1097-1103 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kaneyuki Kubushiro,et.al.: "Effect of gelonin immunoconjugate with monoclonal antibody MSN-1 to endometrial adenocarcinoma" Jpn J Cancer Res. 89. 583-588 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kaneyuki Kubushiro,et.al.: "Glycoconjugates in endometrial cancer" Acta Cytologica. 42. 587-588 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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