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Apoptosis in Human Amnion

Research Project

Project/Area Number 10671576
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionOsaka Medical College

Principal Investigator

OTSUKI Yoshinori  Faculty of Medicine, Osaka Medical College Professor, 医学部, 教授 (50140166)

Co-Investigator(Kenkyū-buntansha) KUMAGAI Koji  Faculty of Medicine, Osaka Medical College Assistant, 医学部, 助手 (40247854)
UEKI Minoru  Faculty of Medicine, Osaka Medical College Professor, 医学部, 教授 (40084892)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
KeywordsHuman amnion / apoptosis / necrosis / Ruptured membrane / Bcl-2 / Fas / Fas ligand / caspase family / TUNEL法 / 透過型電顕 / 卵膜 / ヒト / 免疫組織化学 / 免疫電顕 / TUNEL染色
Research Abstract

This study was designed to detect apoptosis in the human amnion and to elucidate its signalling. Samples of human amnion were obtained from 34 women (11th-42nd week of gestation) and studied usins the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labelling (TUNEL) method by light microscopy (LM) and transmission electron microscopy (TEM), immunohistochemistry, and caspase activitv assay. The TUNEL method by LM demonstrated that the percentage of TUNEL-positive cells in the amniotic epithelium was the highest in the 40th-41st week of gestation (P <0.05) independent of the onset of labour, and the cells were often detached from the epithelium into the amniotic cavity at term. The TUNEL method by TEM clearly showed the characteristic features of apoptosis such as the nuclear condensed chromatin with abundant free 3'-OH DNA ends, cell shrinkage and decrease in the number of desmosomes, except for the presence of apoptotic bodies. Fas and Fas ligand (FasL) were constantly expressed on apical membranes of amniotic epithelial cells through the 16th-27th to 40th-41st week of gestation, while no Bcl-2 expression was observed throughout the gestational periods. Activities of caspase-3 and -8, but not that of caspase-9, were higher in the 40th-41st week than those on the 16th-27th week of gestation (P <0.01). We conclude that apoptosis in term amniotic epithelium is independent of Bcl-2 regulation and onset of labour, and may play an important role in the fragility and rupture of human foetal membranes at term.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Y.Otsuki: "Apoptosis in normal human endometrium"In Proceedings of Oxford-Kobe Seminars (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Kumagai: "Apoptosis in the normal human amnion, independent of Bcl-2 regulation and onset of labour"Mol.Human Reprod. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 大槻勝紀: "子宮内膜におけるアポトーシス・シグナル伝達経路"エンドメトリオーシス研究会誌Vol.22.(印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Otsuki: "Review : Various methods of apoptosis detection."Acta Histochem.Cytochem.. 33. 235-242 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Yamashita: "Fas ligand, Fas antigen and Bcl-2 expression in human endometrium during the menstrual cycle."Mol.Human Reprod.. 5. 358-364 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 大槻勝紀: "総説:アポトーシスとは?"臨床電顕. 32. 5-15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Ueda: "Apoptosis and tumor angiogenesis in cervical cancer after preoperativ chemotherapy."Cancer Res.. 58. 2343-2346 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] E.Daikoku: "The induction of apoptosis in ovaries and uteri of bcl-2-deficient mice."Med.Electron Microsc.. 31. 68-76 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 植木健: "子宮内膜症とアポトーシス"産婦人科の世界. 50. 359-370 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Otsuki: "Molecular Histochemical Techniques"Springer-Verlag. 263 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 大槻勝紀: "臓器別アポトーシス証明法"南江堂. 259 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Otsuki: "Apoptosis in normal human endometrium"In Proceedings of Oxford-Kobe Seminars. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Kumagai: "Apoptosis in the normal human amnion, independent of Bcl-2 regulation and onset of labor"Mol. Human Reprod. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Otsuki: "Review : Various methods of apoptosis detection."Acta Histochem.Cytochem.. 33. 235-242 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Yamashita: "Fas ligand, Fas antigen and Bcl-2 expression in human endometrium during the menstrual cycle."Mol.Human Reprod.. 5. 358-364 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Ueda: "Apoptosis and tumor angiogenesis in cervial cancer after prepoperativ chemotherapy."Cancer Res.. 58. 2343-2346 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] E.Daikoku: "The induction of apoptosis in ovaries and uteri of bcl-2-deficient mice."Med.Electron Microsc.. 31. 68-76 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Otsuki: "Springer-Verlag"Molecular Histochemical Techniques.. 263. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Kumagai: "Apoptotic cascade in term human amnion : mediated by Fas/Fas system and progressed via caspase 8 and 3 dependent pathway."Mol.Human Reprod.. (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Otsuki: "Review : Various methods of apoptosis detection."Acta Histochem.Cytochem.. 33. 235-242 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Yamashita: "Fas ligand, Fas antigen and Bcl-2 expression in human endometrium during the menstrual cycle."Mol.Human Reprod.. 5. 358-364 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 大槻勝紀: "総説:アポトーシスとは?"臨床電顕. 32. 5-15 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 大槻勝紀: "特集:臓器に見られるアポトーシス"電子顕微鏡. 34. 98-101 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] E.Daikoku: "The induction of apoptosis in ovaries and uteri of bcl-2-deficient mice."Med.Electron Microsc.. 31. 68-76 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Otsuki: "Molecular Histochemical Techniques"Springer-Verlag. 263 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 大槻勝紀: "臓器別アポトーシス証明法"南江堂. 259 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Yamashita,Y.Otsuki,Y.Ito,et al.: "Fas ligand expression in human endometrium during the menstrual cycle in relation to Fas antigen and Bcl-2 expressions"Mol.Hum.Reprod.. 5 4. 358-364 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] R.Hayashi,Y.Ito,K.Matsumoto,Y.Fujino and Y.Otsuki: "Quantitative differentiation of both free 3'-OH and 5'-OH DNA ends between heat-induced apoptosis and necrosis"J.Histochem.Cytochem.. 46 4. 783-786 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Ueda,K.Ueki,K.Kumagai,Y.Terai,Y.Okamoto,M.Ueki and Y.Otsuki: "Apoptosis and tumor angiogenesis in cervical cancer after preoperative chemotherapy"Cancer Res.. 58 1. 2343-2346 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 大槻 勝紀: "特集:アポトーシス 「臓器に見られるアポトーシス」"日本電顕学会誌. 34 2. 98-101 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 大槻 勝紀: "総説 アポトーシスとは? -ヒト子宮内膜を例として-"日本電顕学会誌. 32 2. 5-15 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Otsuki: "Mol.Histochemical Techniques"Springer (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 大槻 勝紀: "臓器別アポトーシス証明法"南江堂 (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Ueda: "Apoptosis and tumor angiogenesis in cervical cancer after preoperativchemotherapy." Cancer Res.58. 2343-2346 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] R.Hayashi: "Quantitative differentiation of both free 3'-OH and 5'-OH DNA ends between heat-induced apoptosis and necrosis." J.Histochem.Cytochem.49. 1051-1059 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Ito: "Localization of apoptotic cells inthe human epidermis by an in situ nickend-labeling method using confocal reflectant laser microscopy." J.Histochem.Cytochem.46. 783-786 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] E.Daikoku: "The induction of apoptosis in ovaries and uteri of bcl-2-deficient mice." Med.Electron Microsc.31. 68-76 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 植木健: "子宮内膜症とアポトーシス" 産婦の世界. 50. 359-370 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] C.Inoki: "Image analysis and ultrastructual detection of DNA strand breaks by in situ end-labeling techniques in human endometrium." J.Histotechology. 20. 321-328 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Otsuki: "Molecular Histochemical Techniques" Springer-Verlag, (in press)

    • Related Report
      1998 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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