| Project/Area Number |
10671577
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | KANSAI MEDICAL UNIVERSITY |
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Principal Investigator |
YASUDA Katsuhiko Kansai Med.Univ., Obstetrics & Gynecology, Associate Professor, 医学部, 助教授 (90174507)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Takao Kansai Med.Univ., Obstetrics & Gynecology, Assistant Professor, 医学部, 講師 (10224677)
KANZAKI Hideharu Kansai Med.Univ., Obstetrics & Gynecology, Professor, 医学部, 教授 (80135566)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | PAF / PAF-AH / PAF receptor / Uterine contraction / Pregnancy |
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| Research Abstract |
We studied the role of platelet-activating factor (PAF) in the parturition. PAF caused the uterine contraction in pregnant rat, but did not in the non-pregnant rat. The response to PAF increased during pregnancy and reached the maximum in the late stages of pregnancy. PAF receptor was investigated by using Northern blotting. The expression of mRNA of PAF receptor was observed in both non-pregnant rat and pregnant rat uterus, however, no significant difference in the expression was found between the non-pregnant rat and pregnant rat. The PAP existed in the uterine myometrium and the level of PAF increased during pregnancy and reached the maximum in the late stage of pregnancy. On the other hand, the intracellular PAF acetylhydrolase activity to inactivate PAF decreased during pregnancy and reached the minimum in the late stage. In human uterus, PAF did not cause the uterine contraction in non-pregnant and pregnant women even though the expression of PAF receptor was observed in the uterus in both non-pregnant and pregnant women. No significant difference in the expression was found between both groups. PAF acetylhydrolase activity in the uterus in pregnant women was significantly lower than that in non-pregnant women. When the subunits of intracellular PAF acetylhydrolase isoform I (α1, α2, β) and II in the uterus were investigated by using Western blotting, α2 subunit and β subunit decreased significantly in pregnant women compared with non-pregnant women. α1 subunit was not observed and PAF acetylhydrolase isoform II did not change significantly in both groups.
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