Analysis of the X and Y Chromosomes in Human Spermatozoa Prepared by Swim-up and Percoll Technique
| Project/Area Number |
10671578
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Department of Obstetrics & Gynecology, Kinki University School of Medicine |
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Principal Investigator |
HOSHIAI Hirohsi Kinki University School of Medicine, professor, 医学部, 教授 (50111290)
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| Co-Investigator(Kenkyū-buntansha) |
三橋 洋治 近畿大学, 医学部・附属病院, 講師 (70229748)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2000: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | sperm / Swim-up / Percoll / FISH / aneuploidy / IVF&ET / sperm / sterility / FISH / chromosome anomaly / fetilization / microfertilization / swim up / percoll / 染色体異常 / ART |
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| Research Abstract |
This study has detected whether the selection of motile sperm by Swim-up and Percoll method can fully reflect true genetic quality. We used the twocolor fluorescence in situ hybridization (FISH) technique to simul taneously identify X- and Y-bearing sperm before and after the Swim-up or the Percoll. The samples were ob-tained by masturbation, and prepared for the swim-up and Percoll procedures. Neat semen was served as the control. A total of 21843 sperm in the neat semen (control) were scored. The percentage of Xbearing sperm : Y-bearing sperm was 50.0% : 49.8%, and aneuploidy sperm 0+26%. In the Swirnmp upper layer, 15289 sperm were scored and X : Y sperm was 47.6% : 52.2%, aneuploidy sperm 0.17〜. In the swim-up bottom layer, 20185 sperm were scored and X : Y sperm was 52.2% : 47.3〜, aneuploidy sperm 0.50%. And in the Percoll group 18536 sperm were scored and X : Y sperm was 50.2% : 49.6%, aneuploidy sperm 0.19〜. A significant difference was observed in the swirn, up upper layer fraction and Swirnrup bottom layer fraction (p<0.05). About 3C) 40% of the aneuploidy sperm were removed by the swim-up or the Percoll procedures, but there was about 0.2% aneuploidy sperm existed yet. We should combine certain procedures to obtain true normal sperm for assisted reproductive tech-niques.
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Report
(4 results)
Research Products
(12 results)
