STUDY OF MUCOSAL IMMUNITY AGAINST VIRAL INFECTION IN THE UPPER AIRWAY
| Project/Area Number |
10671608
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
SAITO Tadahito Wakayama Medical University, Assistant Professor, 医学部, 講師 (80205658)
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| Co-Investigator(Kenkyū-buntansha) |
TOGAWA Akihisa Wakayama Medical University, instructor, 医学部, 助手 (70305762)
YAMANAKA Noboru Wakayama Medical University, Professor, 医学部, 教授 (10136963)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | mucosal immunity / upper airway / tonsil / viral infection / co-stimulatory factor / habitual tonsillitis / costimulatory signal |
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| Research Abstract |
The palatine tonsils organize Waldeyer ring and have been thought to serve as inductive site of mucosal immunity of upper airway. The co-stimulatory factors (CD80, CD86) play an important role in the initiation and the maintenance of immune response. In this study, we investigated the expression of the co-stimulatory factor on tonsillar B cells. CD80 and CD86 were constitutively expressed on freshly isolated tonsillar B cells and the expression was higher in tonsils of young aged compared with those of adults. Furthermore, those expression of tonsils affected with recurrent infection (habitual tonsillitis) were significantly lower than those with no episode of infection. However, stimulation of tonsillar B-cells with respiratory syncytial virus (RSV) or staphylococcal enterotoxin B (SEB) up-regulated the expression of the co-stimulatory factors on tonsillar B-cells of tonsils affected with recurrent infection equally with those with no episode of infection. Furthermore, we demonstrated
… More
that increase of the expression of the co-stimulatory factors on tonsillar B-cells needed help of tonsillar T-cells, and the co-stimulatory factor on tonsillar B-cells functionally augmented antigen-specific proliferation of tonsillar T-cellls. Concentration of IL-10 in the supernatant of the culture of the tonsillar mononuclear cells was higher in tonsils affected with recurrent infection than those with no episode of infection. It seems that IL-10 had tendency to suppress the expression of the co-stimulatory factors on tonsillar B-cells. We also showed by the intra-cellular cytokine staining technique that the IL-10-producing cells were mainly T-cells. Together with these observations, our results imply that Il-10 produced by tonsillar T cells inhibits the expression of co-stimulatory factors of B cells in habitual tonsillitis. In conclusion, we report that co-stimulatory factors on tonsillar B-cells play important roles on the immune responses of tonsils and the pathogenesis of habitual tonsillitis is the state of impaired immune response against viral or bacterial infection because of low expression of the co-stimulatory factors on tonsillar B-cells. Less
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Report
(3 results)
Research Products
(12 results)
