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STUDY OF PATHOGENESIS IN AUTOIMMUNE INUJRY OF THE INNER EAR

Research Project

Project/Area Number 10671621
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionNIPPON MEDICAL SCHOOL

Principal Investigator

TOMIYAMA Shunichi  NIPPON MEDICAL SHOOL, DEPARTMENT OF OTOLARYNGOLOGY, ASSOCIATE PROFESSOR, 医学部, 助教授 (00094665)

Co-Investigator(Kenkyū-buntansha) 池園 哲郎  日本医科大学, 医学部, 助手 (80277491)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsINNER EAR / AUTOIMMUNE / immunohistchemistry / CD4 / IL-2 / IFN-γ / western blot / autoantibody / Th1 / 血清自己抗体 / 細胞傷害性リンパ球 / 表面抗原 / マウス / モデル動物 / 自己反応性リンパ球 / 迷路炎
Research Abstract

This study was designed to establish an experimental autoimmune labyrinthitis model in inbred mouse, which could exhibit high reproducibility and be adopted for precise immunological analysis. Following pretreatment with cyclophosphamide and primary sensitization with inner ear antigen (IEA) in FCA, many inflammatory cells transiently infiltrated into the whole area of the inner ear. This reaction occurred in all animals and peaked on day 12. This labyrinthitis was passively transferred by sensitized lymphocytes. Many CD4+ cells with IFN-γ+ and IL-2+ infiltrated mainly the endolymphatic sac as early as day 4, gradually into the whole area of the inner ear and persisted until day 35. On the other hand, very few CD8+ cells infiltrated on day 10 and rapidly disappeared. These results suggested that primary sensitization induce cellular autoimmune reaction in the inner ear mediated by helper T1 (Th1 ) lymphocytes. Repeated sensitization with IEA produced autoantibody to the inner ear. Western blot assay demonstrated three different serum antibodies, which reacted to 45, 66, 200 KD of the bovine inner ear antigen. IgG deposited mainly in the vessels of the stria vascularis and bone labyrinthine. Repeated sensitization with IEA stimulates humor mediated autoimmune reaction and autoantibody may alter morbid state of chronic stage of autoirmmune labyrinthitis. Immune reaction in the endolymphatic sac generates immune injury by nitric oxide, apoptotic degeneration, as well as sympathetic labyrinthitis.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] 高橋光明: "Eaモット内リンパ嚢の抗原排除能"日耳鼻. 101. 1089-1092 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 高橋光明: "内リンパ水腫作成動物の対側内耳の変化"Otol Jpn. 8. 521-525 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 富山俊一: "内耳自己免疫病"アレルギーの臨床. 19. 429-433 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomiyama Shunichi: "Experimental autoimmune labyrinthitis induced by cell-mediated immune reaction"Acta Otolaryngol (Stockh). 119. 665-670 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikezono Tekurou: "Passive transfer of the experimental autoimmune labyrinthitis."Audiolo Neurootol. 5. 292-299 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomiyama Shunichi: "Th1 : mediator lymphocytes in experimental autoimmune labyrinthitis"Acto Otolaryngol (Stockh). (in print). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Harris JP: "Meniere's Disease"Kugler publication. 418 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomiyama Shunichi: "New frontiers in immune biology"Kugler publication. 280 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Takahashi: "Antigen removal after inoculation into the endolymphatic sac of immunized guinea pigs."J Otolaryngol Jpn. 101. 1088-1092 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Takahashi: "Change of contralateral inner ear after endolymphatic sac immune injury."Otol Jpn. 8. 521-525 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Tomiyama: "Autoimmune inner ear disease."The allergrgy in practice. 19. 429-433 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Tomiyama: "Experimental autoimmune labyrinthitis induced by cell-mediated immune reaction."Acta Otolaryngol (Stockh). 119. 665-670 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Ikezono: "Passive transfer of the experimental autoimmune labyrinthitis."Audiolo Neurootol. 5. 292-299 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Watanabe: "Detection of single-DNA in the hydropic vestibule after the direct injection of antigen into the endolymphatic sac of guinea pigs."Neurosci Lett. 293. 57-60 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Tomiyama: "Th1 ; Mediator lymphocytes in experimental autoimmune labyrinthitis."Acta Otolaryngol. (Stockh)120(in print). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Watanabe: "Expression of inducible nitric oxide synthase (iNOS/NOS II) in the cochlea following immune response in the endolymphatic sac of ginea pigs."ORL. 63(in print). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] JP.Harris: "Kulger publication, Meniere's disease"418 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Tomiyama: "Kulger publication, New frontiers in immunobiology"280 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I Kezono T: "Passive transfer of the experimental autoimmune labyrinthitis"Audiolo Neurootol. 5. 292-299 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Watanabe K: "Detection of Single-DNA in the hydropic vestibule after the direct injection of antigen into the endolymphatic sac of guinea pigs"Neurosci Lett. 293. 57-60 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tomiyama S: "Thl : Mediator lymphocytes in experimental autoimmune labyrinthitis"Acta otolaryngol (Stoclch). (in print). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Watanabe K: "Expression of inducible nitric oxide synthase (iNos/NosII) in the cochlea following immune response in the endolymphatic sac of guinea pigs"ORL. (in print). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tomiyama S.: "New frontiers in immunobiology."Veld man JE.Passali D.Lim DJ.. 280 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tomiyama S: "Experimental autoimmune labyrinthitis mediated by cell-mediated immune reaction.."Acta Otolaryngol(Stockh). 119. 665-670 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Harris JP: "Meniere's disease"Harris JP. 418 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 高橋光明 富山俊一: "モルモット内リンパ癌の抗原排除能" 日耳鼻. 101. 1088-1092 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 高橋光明 富山俊一: "内リンパ水腫作製動物の対側内耳の変化" Otol JPN. 8. 521-525 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 富山俊一: "内耳自己免疫病" アレルギーの臨床. 印刷中. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tomiyama.S. et al: "Immuno-and mdecular Biology in ORL-State of ART" Kulger Publication (印刷中), (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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