| Project/Area Number |
10671630
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
ABE Toshiaki Department of Ophthalmology, Tohoku University, Associate Professor, 大学院・医学系研究科, 助教授 (90191858)
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Hiroshi Department of Ophthalmology, Tohoku University, Research Associate, 大学院・医学系研究科, 助手 (40302088)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | ischemia / transplantation / iris pigment epithelial cells / brain-derived nerve growth factor / retina / rejection / サイトカイン / 脳由来神経成長因子(BDNF) / 虹彩色素上皮細胞(IPE) / 遺伝子導入 / アマクリン細胞 / 網膜下移植 |
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| Research Abstract |
Diabetic retinopathy (PDR) is one of the famous ischemic retinal disease and age-related macular degeneration (AMD) is also suspected to have hypoperfusion area of the choroid at the background condition. These ischemic retinal disease show main cause of blindness in the world. We have examined several cytokine gene expression in the proliferative membranes in patients with AMD, proliferative vitreoretinopathy (PVR), and PDR. Comparison of the gene expression with each other demonstrated that some cytokines such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor manifested high expression in these lesions. Brain-derived nerve growth factor (BDNF) also demonstrated in these membranes. On the other hand, BDNF is reported to show nerve protective effect against the experimentary induced ischemic condition of the retina. The protective effect is prominent in the inner region of the retina in the condition, especially the ganglion cells and amacrine cells. We performed permanent BDNF gene transfection in the rat iris pigment epithelial cell. The transfected cell was used for transplantation and ischemic study in the rats. As far as we examined, the transplanted rats showed not rejection sign and protective effect against ischemic experiment, although the experimental number was not enough for statistical analysis so far. From these experiment, our methods using iris pigment epithelial cells, which is easy to obtain from individuals, may have potential s not only for transplantation study against ischemic retinal disease but for other retinal diseases.
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