| Project/Area Number |
10671634
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Tokyo University |
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Principal Investigator |
NUMAGA Jiro Tokyo University, Faculty of Medicine Branch Hospital, Assistant Prof., 医学部・附属病院・分院, 講師 (30189352)
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| Co-Investigator(Kenkyū-buntansha) |
JOKO Satoru Tokyo University, Faculty of Medicine Branch Hospital, Lecturer, 医学部・附属病院・分院, 助手 (80246470)
FUJINO Yujiro Tokyo Kouseinenkin Hospital, Assistant Prof., 眼科部長(研究職) (30143465)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | retinal pigment epithelial cell / RPE protective protein / uveitis / endotoxin / lipid peroxidation / ornithine induced retinopathy / tissue damage |
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| Research Abstract |
We isolated and characterized a novel protein secreted by cultured retinal pigment epithelial cells (RPE). This protein, which we proposed to name RPE protective protein (RPP), suppresses superoxide production in activated phagocytes in vitro. This study was designed to investigate the in vivo anti-inflammatory effect of RPP and the effcets on ornitine induced reinopathy.
Exp 1
Rabbits eyes with endotoxin induced uveitis were injected intravitreally with crude and inactivated RPP. Twenty-four hours after injection, aqueous humor and vitreous samples were analyzed for protein concentrations, cells numbers, and lipid peroxidation extracted from iris-ciliary body, choroid and retina. The decrease in superoxide and protein contents in vitreous samples injected with crude RPP was statistically significant compared to eyes injected with inactivated RPP. Lipid peroxidation were significantly decreased in the retinas. However, there was no significant difference in the number of infiltrating cells in aqueous humor and vitreous samples. RPP have the anti-inflammatory effects to rescue the tissue damage.
Exp 2
L-ornithine hydrochloride (0.25M/1 or 0.5M/1) were injected into vitreous body capacities and RPE-CS was injected in treated groups, and heat inactivated RPP in control groups, together. After 5 days, the eye balls were enucleated and investigatived histopathologically. RPE and outer retinal segment were received damage in controls as previous reports. On the other hand, however, there were light damage in treated groups. As the mechanism of this effects was not specialized, RPP has the effects of decreasing the tissue damage induced by ornithine.
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