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Possibilities of Organs Growth and Maturation of Fetal Goat Incubated in Artificial Amniotic Fluid (Artificial Placenta)

Research Project

Project/Area Number 10671670
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatric surgery
Research InstitutionKobe University

Principal Investigator

HISANO Katsuya  Kobe University School of Medicine, Surgery, Associate Professor, 医学部, 助教授 (30135800)

Co-Investigator(Kenkyū-buntansha) UETANI Yosiyuki  Kobe University School of Medicine, Pediatrics, Associate Professor, 医学部, 助教授 (40168620)
OBARA Hidesi  Kobe University School of Medicine, Anesthesiology, Professor, 医学部, 教授 (80030998)
安福 正男  神戸大学, 医学部附属病院, 医員
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsartificial placenta / membrane oxygenator / ECMO / organ maturation / Artificial placenta / fetal development / 遠心ポンプ / 胎児手術 / fetal surgery
Research Abstract

Eight goat fetuses at about 125 days' gestation (term, 150 days) were placed in the fetal ECMO system. Their four twin fetuses with similar body weights were harvested and examined as baseline data before ECMO (pre-ECMO data). The mean duration of A-V ECMO was 138.8 hours (range : 87 to 237hours). Medication of organ growth factor such as steroid hormone, thyroid hormon and epitherial growth factor was performed on all four fetuses. The lung weights of the four fetuses with tracheal ligation are compared. The surfactant and electrolytes of the tracheal fluid were analyzed in pre-ECMO (twin fetuses) and during ECMO. The surfactant of lung tissue, and lung weight were analyzed in pre-ECMO (twin fetuses) and after ECMO. Plasma cortisol and T3 levels were also assayed as hormonal factors that effected lung maturation in pre-ECMO and during ECMO.
In conclusion, this fetal A-V ECMO systems in the bath showed a production of surfactant, the maintenance of ion transport by the pulmonary epithelium, an increase in lung weight, and an increase in mature type II cells, resulting in lung growth and maturation. he lungs of all four fetuses were weighed after ECMO. Both wet and dry lung weight were significantly higher after ECMO than pre-ECMO. The wet lung, brain and liver weights as proportions of fetal body weights were higher after ECMO, but not significantly differents from pre-ECMO.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 安福正男,久野克也 他: "胎児ECMOにおける気管結紮の肺成長促進効果"日小外会誌. 34. 68-75 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 岡 成光,久野克也 他: "遠心ポンプ駆動によるV-V ECMOシステムの開発と臨床応用"神戸大学医学部紀要. 59. 101-107 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 久野克也 他: "小児重症呼吸不全の医工学治療 ーECMOー"医工学治療. 11. 448-450 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasufuku, M., Hisano, K., et al.: "Arterio-venous extracorporeal membrane oxygenation of fetal goat incubated in artificial amniotic fluid (artificial placenta) : Influence on lung growth and maturation"J. Pediatr Surg.. 33. 442-448 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakata, M., Hisano, K., et al.: "A new artificial placenta with a centrifugal pump : Long term total extrauterine support of goat fetuses"J. Thorac Cardiovasc Surg. 115. 1023-1031 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Pokharel, R., Hisano, K., et al.: "Ligation of medically refracted patent ductus arteriosus (PDA) in an extremely low body weight premature infant"Surg. Today. 28. 1290-1294 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasuhuku M, Hisano K et.al.: "Arterio-venous extracorporeal membrane oxygenation (A-V ECMO) via the umbilical vessels of goat fetuses : The effect of tracheal occlusion"J. Jan. Soc. Pediatr. Surg.. 34. 800-807 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasuhuku M, Hisano K et.al.: "Arterio-venous extracorporeal membrane oxygenation of fetal goat incubated in artificial amniotic fluid (artificial placenta) : Influence on lung growth and maturation"J Pediatr Surg. 33. 442-448 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakata M, Hisano K et.al.: "A new artificial placenta with a centrifugal pump : Lung term total extrauterine support of goat fetuses"J Thorac Cardiovasc Surg. 115. 1023-1031 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Pokharel R, Hisano K et.al.: "Ligation of medically refracted patent ductus arteriosus (PDA) in an extremely low body weight premature infant"Surg Today. 28. 1290-1294 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oka N, Hisano K et.al.: "Development and clinical application of new venovenous extracorporeal membrane oxygenation system using a centrifugal pump"Medical J of Kobe University. 59. 101-107 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hisano K, Okada M et.al.: "Medical engineering therapy for severe pediatric respiratory failure"-ECMO- Therapeutics and Engineering. 11. 448-450 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 久野克也 他: "小児重症呼吸不全の医工学治療 -ECMO-"医工学治療. 11. 448-450 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 岡成光,久野克也 他: "遠心ポンプ駆動によるV-V ECMOシステムの開発と臨床応用"神戸大学医学部紀要. 59. 101-107 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Sakata,K.Hisano: "A new artificial placenta with a centrifugal pump:Long-term total etrauterine support of goat fetuses" J Thorac Cardiovasc Surg. 115. 1023-1031 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Yasufuku,K.Hisano: "Arterio-venous extracorporeal membrane oxygenation of fetal goat incubated in artificial amniotic fluid(artificial placenta):a possibility of lung growth and maturation" J Pediatric Surg. 33. 442-448 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 久野克也、岡田昌義: "21世紀への人工臓器" 岡田昌義、安田慶秀 先端医療技術研究所, 332 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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