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ELUCIDATION OF THE MOLECULAR MECHANISM OF KELOIDS AND HVPERTROPHIC SCARS USING SPECIFIC MOLECULAR PROBES

Research Project

Project/Area Number 10671680
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionKAGAWA MEDICAL UNIVERSITY

Principal Investigator

KOBAYASHI Ryoji (1999)  Kagawa Medical University, Faculty of Medicine, Professor, 医学部, 教授 (00020917)

獅々堀 彊 (1998)  香川医科大学, 医学部, 助教授 (10033870)

Co-Investigator(Kenkyū-buntansha) SHISHIBORI Tsuyoshi  Kagawa Medical College, Professor, 臨床検査学科, 教授 (10033870)
HATA Yuiro  Kagawa Medical University, Faculty of Medicine, Professor (90164839)
小林 良二  香川医科大学, 医学部, 教授 (00020917)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsextracellular matrix protein / elastin / 36-kDa MAGP / microfibril / tranilast / molecular pharmacology / drug affinity chromatography / 36kDaMAGP / エラスチン繊維 / 細胞接着 / Smith-Magenis症候群 / ミクロフィブリル / 細胞外マトリックス蛋白質 / RGD蛋白質 / アフィニティクロマトグラフィー
Research Abstract

To elucidate the molecular mechanism involved in the suppression of keloids and hypertrophic scars by tranilast, we investegated the target proteins of tranilast and other anti-allergic drugs in bovine skin and aorta. A specific tranilast-binding protein was isolated from both tissues by drug affinity chromatography and was identified as 36-kDa microfibril-associated glycoprotein (36-kDa MAGP). Binding of 36-kDa MAGP to tranilast seemed to be specific since 36-kDa MAGP could be eluted from the drug affinity column by tranilast itself and also binding of 36-kDa MAGP to other anti-allergic drugs, such as cromolyn and amlexanox, is significantly weaker than that to tranilast. Light and electron microscopic immunohistochemistry detected the protein at the periphery of elastic fibers in normal human skin. In hypertrophic scar tissue, however, 36-kDa MAGP was located on small bundles of microfibrils. These findings provide support for te concept that elastogenesis occurs in scar tissue and 36-kDa MAGP might be one of the target for tranilast. Based on the evidence, we synthesized several compounds which specifically bind to 36-kDa MAGP.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kayoko Yamashita et al: "Purification of Bovine S100A12 from Recimbinant Escherichia coli"Protein Expression and Purification. 16. 47-52 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuyoshi Shishibori et al: "Three distinct anti-allergic drugs,amlexanox,cromolyn and tranilast,bind to S100A12 and S100A13 of the S100 protein family"Biochem.J.. 338. 583-589 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuhiko Toyoshima et al: "Ultrastructual distibution of 36-kD Microfibril-associated Glycoprotein (MAGP-36) in human and Bovine Tissues"The Journal of Histochemistry&Cytochemistry. 47. 1049-1056 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiromi Furuichi et al: "Identification of Tranilast-binding protein as 36-kDa microfibril-associated glycoprotein by affinity chromatography,and localization in human skin"Biochem.Biophys.Res.Commun.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shishibori T, Oyama Y, Matsushita O, Yamashita K, Furuichi H, Okabe A, Maeta H, Hata Y and Kobayashi, R: "Three distinct anti-allergic drugs, amlexanox, cromolyn and tranilast, bind to S 100A12 and S 100A13 of the S 100 protein family"Biochem J. 338. 583-589 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toyoshima T, Yamashita K, Furuichi H, Shishibori T, Itano T and Kobayashi R: "Ultrastructural distribution of 36-kDa microfibril-associated glycoprotein (MAGP-36) in human and bovine tisuess"J Histochem Cytochem. 47. 1049-1056 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamashita K, Oyama Y, Shishibori T, Matsushita O, Okabe A and Kobayashi R: "Purification of bovine S100A12 from recombinant Escherichia coli"Protein Expr Purif. 16. 47-52 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Furuichi H, Yamashita K, Okada M, Toyoshima T, Hata Y, Suzuki S, Itano T, Shishibori T, Tokumitsu H and Kobayashi R: "Identification of tranilast-binding protein as 36-kDa microfibril associated glycoprotein by drug affinity chromatography, and its localization in human skin"Biochem Biophys Res Commun. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kayoko Yamashita et al: "Purification of Bovine S100A12 from Recombinant Escherichia coli."Protein Expression and Purification. 16. 47-52 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuyoshi Shishibori et al: "Three distinct anti-allergic drugs,amlexanox,cromolyn and tranilast,bind to S100A12 and S100A13 of the S100 protein family."Biochem.J.. 338. 583-589 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tetsuhiko Toyoshima et al: "Ultrastructual distribution of 36-kD Microfibril-associated Glycoprotein(MAGP-36)in human and Bovine Tissues."The Journal of Histochemistry&Cytochemistry. 47(8). 1049-1056 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hiromi Furuichi et al: "Identification of Tranilast-binding protein as 36-kDa microfibril-associated glycoprotein by affinity chromatography,and its localization in human skin."Biochem.Biophys.Res.Commun. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Shishibori T.: "Three distinct anti-allergic drugs,amlexanox,cromolyn and tranilast,bind to S100A12 and S100A13 of the S100 protein family." Biochem J.338. 583-589 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamashita K.: "Purification of Bovine S100A12 from Recombinant Escherichia coli." Protein Expr.Purif.(in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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