| Project/Area Number |
10671720
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Meikai University (2000)
Nihon University (1998-1999) |
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Principal Investigator |
OCHIAI Kuniyasu Meikai University, School of Dentistry, Professor., 歯学部, 教授 (50095444)
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| Co-Investigator(Kenkyū-buntansha) |
KURITA-OCHIAI Tomoko Nihon University, School of Dentistry at Matsudo, Assistant professor., 松戸歯学部, 講師 (20130594)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | IL-1β / periodontopathic bacteria / Actinobacills / Prevotella / Porphyromonas / 歯周病 / 歯周病原生菌 / 歯周病原性菌 |
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| Research Abstract |
Interleukin-1β (IL-1β) is a polypeptide cytokine that mediates many physiological to infection and inflamation and is a growth factor for certain mamalian cells. We revealed that certain periodontopathic bacteria produce unknown substance which react with mouse anti-human IL-1β antibody. Therefore, we examined the poduction of IL-1β like substance by periodontopathic bacteria, such as Actinobacills, Prevotella, Porphyromonas, Capnocytophaga, Fusobacterium. ELISA results clearly revealed that Actinobacills actinomycetemcomitans, Prevotella intermedia and Prevotella nigrescence produce IL-1β like substance. Amino acid analysis from N-terminal of affinity purified this substances were not a simillar as human IL-1β. The differensis were, also, foud form the analysis of RT-PCR products. Further investigation is required in antigenic site on IL-1β like substance.
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