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Effects of bone morphogenetic protein-2 and transforming growth factor-β1 on gene expression of decorin and biglycan by cultured osteoblastic cells.

Research Project

Project/Area Number 10671721
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionNihon University

Principal Investigator

TAKAGI Minoru  Nihon University, School of Dentistry, Professor, 歯学部, 教授 (90060061)

Co-Investigator(Kenkyū-buntansha) URUSHIZAKI Toshiko  Nihon University, School of Dentistry, Research Associate, 歯学部, 助手 (80297845)
KAMIYA Naoko  Nihon University, School of Dentistry, Research Associat, 歯学部, 助手 (80287656)
SHIGEMASA Kayoko  Nihon University, School of Dentistry, Research Associate, 歯学部, 助手 (30059604)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsosteoblasts / cell culture / BMP / proteoglycan / decorin / biglycan / alkaline phosphatase / TGF-β / biglycan
Research Abstract

The influence of bone morphogenetic protein-2 (BMP-2) and transforming growth factor β (TGF-β) on the expression of small proteoglycans, decorin and biglycan was investigated in a clonal rat osteoblastic cell line, ROS-C26 (C26) cells, which is a potential osteoblast precursor cell line and capable of differentiating into mature osteoblasts after treatment with recombinant BMP-2 (rhBMP-2). Following the culture of C26 cells for 3, 6, and 9 days in the presence or absence of rhBMP-2, alkaline phosphatase activity increased in the rhBMP-2 treated cells in direct proportion to their differentiation into more mature osteoblastic cells, whereas decorin mRNA decreased in the cells, when compared to control cells without rhBMP-2 treatment. These results were evident 6 days after treatment. However, rhBMP-2 treatment had no effect on biglycan mRNA expression in the cells. Subsequently, after removal of rhBMP-2 from the culture media, the cells were further cultured for 24 h with graded concentrations of TGF-β1 (0,0.1,1.0, 5.0, and 10 ng/ml). TGF-β1 decreased decorin mRNA expression in the cells dose dependently, but did not effect their biglycan mRNA expression. Furthermore, either removal of rhBMP-2 from the culture media or addition of TGF-β1 significantly decreased alkaline phosphatase activity of rhBMP-2-induced cells. There results indicate that osteoblastic differentiation is accompanied by increased alkaline phosphatase activity and decreased expression of decorin mRNA, but continuous expression of biglycan mRNA. Both rhBMP-2 and TGF-β1 inhibit decorin mRNA expression in osteoblasts at varying stages of differentiation, but their effects on biglycan mRNA expression and alkaline phosphatase are different.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Takagi, M. et al.: "Effects of bone morphogenetic protein-2 and transforming growth factor-β1 on gene expression of decorin and biglycan by cultured osteoblastic cells"Histochem.J.. 31. 403-409 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada, T., Takagi, M. et al.: "Effects of transforming growth factor-β1 on the gene expression of decorin, bigycan, and alkaline phosphatase in osteoblast precursor cells and more differentiated osteoblast cells"Histochem.J.. 31. 687-694 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takagi, M., Yamada, T., Kamiya, N., Kumagai, T. and Yamaguchi, A.: "Effects of bone morphogenetic protein-2 and transforming growth factor-β1 on gene expression of decorin and biglycan by cultured osteoblastic cells."Histochem. J.. 31. 403-409 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takagi,M.et al.: "Effects of bone morphogenetic protein-2 and transforming growth factor-β1 on gene expression of decorin and biglycan by cultured osteoblastic cells."Histochem.J.. 31・6. 403-409 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamada,T.,Takagi,M.et al.: "Effects of transforming growth factor-β1 on the gene expression of decorin,biglycan,and alkaline phosphatase in osteoblast precursor cells and more differentiated osteoblast cells."Histochem.J.. 31・10. 687-694 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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