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Molecular mechanisms of cleft palate formation in knockout mice

Research Project

Project/Area Number 10671723
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

TAYA Yuji  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Lecturer, 歯学部, 講師 (30197587)

Co-Investigator(Kenkyū-buntansha) SHIMAZU Yoshihito  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Assistant Professor, 歯学部, 助手 (10297947)
SATO Kaori  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Assistant Professor, 歯学部, 助手 (90287772)
YAGISHITA Hisao  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Lecturer, 歯学部, 講師 (50256989)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordssecondary palatogenesis / knockout mice / medial edge epithelium / deltaEF-1 / endothelin / cell adhesion / programmed cell death / proteoglycan / 口蓋形成 / 口蓋突起上皮(MEE細胞) / TGFβ / delta EF-1 / 糸状仮足
Research Abstract

Secondary palatogenesis in the mammal provides an excellent experimental model to investigate the mechanism of embryonal morphogenesis comprising cell proliferation and differentiation, and their movement and extinction at specified developmental stages. In the present work using mouse embryos (C57BL/6 and ICR), we focused our attention on the temporo-spatial changes in tissue organization composing of epithelial and messenchymal cells throughout palatogenesis. The critical event prior to completion of secondary palatogenesis required differentiation of the epithelial cell population into the medial edge epithelial (MEE) cells, which correspond to approximately 4,000 cells in a pair of the shelves. Filopodia on the MEE cell surface mediated an initial contact between the opposing shelves. After the cellular contact, the high mobility of MEE cells facilitated construction of the midline epithelial seam, which eventually became discontinuous and disappeared prior to mesenchymal confluenc … More e. Regarding the fate of the MEE cells at the midline position of mouse secondary palate, our results support the concomitant occurrence of programmed cell death at low frequencies, epithelial-mesenchymal transformation, and cell migration to join with oral or nasal epithelium. We also examined the structural changes and migration of MEE (medial edge epithelial) cell during the fusion of mouse embryonic palatal shelves in vitro. The palatal shelves used were prelabelled by CCFSE (carboxy-dichlorofluorescein diacetate succinimidyl ester) for 30 min at room temperature. The results obtained from the cultured shelves revealed that : (a) selective CCFSE- labeling was attained in the MEE cells, (b) a large number of the CCFSE-labeled MEE cells were clustered in the epithelial triangles, thereafter shedding into the cavities along the midline seam, and c only a few labeled cells at disintegrating seams appeared to merge into the mesenchymal cells. The overall results support the concept that the MEE cells are destined for discrete terminations during palatogenesis. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 藤原美恵子、他: "マウス二次口蓋形成における突起尖端上皮細胞の動態と表現型の経時的推移"歯科基礎誌. 42.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 田谷雄二、他: "マウス二次口蓋形成と遺伝子改変マウスに発症する口蓋裂"歯科基礎誌. 41. 531-539 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Taya, et al.: "Pathogenesis of cleft palate in TGF-β3 knockout mice."Development. 126. 3869-3879 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 添野雄一、他: "In vitoroにおけるマウス胎仔口蓋突起上皮の癒合・消失:細胞蛍光標識法の応用"歯学. 87. 44-50 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Fujiwara, et al.: "Cellular dynamics and phenotypic modulation of palatal shelves-lining epithelium during mouse secondary palatogenesis."Jpn J Oral Biol.. 42 (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Taya, et al.: "Palatogenesis and Cleft Palate Formation in Mice : Related Genes and Molecules."Jpn J Oral Biol.. 41. 531-539 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Taya, et al: "Pathogenesis of cleft palate in TGF-β3 knockout mice."Development. 126. 3869-3879 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Soeno, et al.: "The Fate of MEE Cells of Mouse Embryonic Palatal Shelves in Vitro : Use of Carboxyfluorescein Labeling."Odontology. 87. 44-50 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 田谷雄二、他: "マウス二次口蓋形成と遺伝子改変マウスに発症する口蓋裂"歯科基礎誌. 41 6. 531-539 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Taya, et al.: "Pathogenesis of cleft palate in TGF-β3 knockout mice."Development. 126. 3869-3879 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 添野雄一、他: "In vitroにおけるマウス胎仔口蓋突起上皮の癒合・消失:細胞蛍光標識法の応用"歯学. 87・1. 44-50 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田谷雄二、他: "ビスフォスフォネート(HEBP)全身投与によるラット臼歯槽部での歯質・歯槽骨の癒着現象と投与中止後における歯周組織の可逆的修復現象" 歯学. 86・3. 616-622 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yuji Taya, et al.: "Pathogenesis of cleft palate in TGF-β3 knockout mice.(preparation)"

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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