| Project/Area Number |
10671727
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Asahi University School of Dentistry |
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Principal Investigator |
KOGAYA Yasutoku Asahi University School of Dentistry, Associate Professor, 歯学部, 助教授 (30076046)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Enameloid / Type Collagen / Odontoblasts / Amelogenin / Hybridization / Gene Expression / Tooth Germs / Newt / エナメル質 / コラーゲン / 硫酸化複合糖質 / insituハイブリダイゼーション / プローブ / 石灰化 |
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| Research Abstract |
Ectoderm-derived hard tissues such as enamel, enameloid(produced jointly by epithelial and ectomesenchymal cells) and ganoine form an external highly mineralized layer on the teeth and scales respectively. The presence of collagen in enameloid distinguishes it clearly from true enamels, but little is known about phylogenetic relationship between these two tissues. The cellular source of enameloid collagen, however, has been remained controversial. With these considerations in mind we searched for collagen and amelogenin genes and characterized the origin of enameloid collagen. Messenger RNA encoding procollagen α 1 type I have been localized using digoxigenin-labelled synthetic oligonucleotide probes. The probe-mRNA hybrides were visualized using an anti-digoxin antibodies. Intense hybridization signals for the procollagen were detected in the fibroblasts of the oral mucosa connective tissues and perichondrium. Further, the positive hybridization signals were also observed in odontoblasts of the tooth germs of the larval urodele (Triturus pyrrhogaster) during enameloid matix formation. But no signals were detected in the oral mucosa epithelial cells including inner epithelial cells. It is highly suggestive that odontoblasts synthesize procollagen α 1 type I of the enameloid matrix.
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