• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of inhibitor of osteoclastogenesis

Research Project

Project/Area Number 10671734
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

MORITA Ikuo  Tokyo Medical and Dental University Graduate School Associate Professor, 大学院・医歯学総合研究科, 助教授 (60100129)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsOsteoclast / osteoclasogenesis / inhibitor of osteoclastogenesis / pit formation / Il-4 / Il-10 / 骨芽細胞 / ODF / 破骨細胞 / インターフェロン
Research Abstract

The purpose of this research was to purify and identify inhibitors of osteoclast formation released from mouse stromal cells (pre-osteoblasts, TMS-12). To quantify the activity of inhibition of osteoclast formation, we developed the assay system for osteoclast formation, and to purify the inhibitors we determined the cultured condition of TMS-14 without serum. Using this system, we carried out the purification of inhibitors from the 120 liter of the conditioned medium of TMS-14 with ion-exchangers, molecular cutting, dialysis and so on. Finally after applying Superdex 200HR10/30 it was determined that the molecular of the inhibitors was 200K and 22K. Now, we carry out the cloning of this substances. Next, we characterized the inhibitors. As a result, the inhibitors were characterized as follows ; 1) It was determined the inhibitors was not IL-4, IL-10, TNFα, known-inhibitors of osteoclast formation by the experiments of antibody neutrization, 2) The substances inhibited osteoclast differentiation, but not proliferation of osteoclast progenitor cells, 3) The substances also changed the morphology of mature osteoclasts and inhibited the pit formation.
These data indicated that the characterization of the inhibitors of osteoclast formation was quietly different from other inhibitors such as osteoprotegrin/osteoclastogenesis inhibitory factor.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Gao Y.Morita I et al.: "Expression of IL-6 receptor and GP130 in mouse bonemarrow cells during osteoclast differentiation"Bone. 22. 487-493 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sato T.Morita I. et al.: "Involvement of cholesterol in osteoclast-like cell formation via cellular fusion"Bone. 23. 135-140 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakaguchi K,Morita I et al.: "Relationship between the ability to support differentaition of osteoclast-like cells and adipogenesis in murine stromal cells derived from bone marrow"PG. LT and EFA. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Gao Y. Morita I. Maruo N. Kubota t. Murota S. Aso T.: "Expression of IL-6 receptor and GP130 in mouse bone marrow cells during osteoclast differentiation."Bone. 22(5). 487-493 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sato T. Morita I. Murota S.: "Involvement of cholesterol in osteoclast-like cell formation via cellular fusion."Bone. 23(2). 135-140 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakaguchi K, Morita I, Murota S: "Relationship between the ability to support differentiation of osteoclast-like cells and adipogenesis in murine stromal cells derived from bone marrow."Prostaglandins, Leukotrienes and Essential Fatty Acids. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakaguchi K, Morita I, Murota S: "Relationship between the ability to support differentaition of osteoclast-like cells and adipogenesis in murine stromal cells derived from bone marrow."Postaglandins,Leukotrienes and Essential Fatty Acids. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Sato: "Invdvemncnt of clulesterol in Ostealast-like Cell Foration via Cellular Fusion" Bone. 23. 135-140 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Gao: "Expression of IL-6 Receptor and GP130 in Mouse Bnu Morra cells During Osteoclast Differetiolin" Bone. 22. 487-493 (1998)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi