| Project/Area Number |
10671755
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | The Nippon Dental University |
|---|
Principal Investigator |
IWABUCHI Yoshiki The Nippon Dental University at Niigata Department of Dental Pharmacology Associate Professor, 新潟歯学部, 助教授 (80095067)
|
|---|
| Project Period (FY) |
1998 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Keywords | Submandibular Gland / Fluid Secretion / Protein Secretion / Neuropeatide Y / VIP / Substance P / SNI-2011 / Pilocarpine / 修飾作用 / ベタネコール / 相互作用 / 糖蛋白 / セクレチン / 電気泳動 |
|---|
| Research Abstract |
This study was carried out to elucidate the role of neuromodulators on the secretion of fluid and protein from salivary gland cells. 1 : Effects of neuropeptide Y on the secretion of fluid and protein from rat submandibular gland induced by the combination of substance P and VIP (vasoactive intestinal peptide) : (1) Substance P elicited large volumes of saliva whereas neuropeptide Y and VIP did not elicit saliva. (2) VIP potentiated the substance P-mediated secretion of fluid and protein. (3) Neuropeptide Y did not affect SP-mediated secretion of fluid. (4) The potentiating effect of secretion of fluid and protein and the long lasting effect of fluid was reduced by neuropeptide Y in a dose- dependent manner. 2 : Subtypes of adrenergic and muscarinic receptors mediating secretion of fluid and protein from rat submandibular gland induced by SNI-2011 and pilocarpine. (1) Salivation on early stage after administration of SNI-2011 and pilocarpine caused through stimulation of muscarinic M_1 receptors in the superior cervical ganelion. (2) Sialogogic effect induced by stimulation of muscarinic M_1 receptors was higher pilocarpine than SNI-2011. (3) Sialogogic action on last stage after administration of SNI-2011 involves stimulation of M_3 receptors and its action was higher than pilocarpine. (4) SNI-2011 and pilocarpine-mediated secretion of fluid was enhanced by VIP.These results sugest that neuropeptide Y and VIP act as neuromodulator on neurotransmitter, tachykinin and muscarinic agents-mediated secretion of fluid and protein from salivary gland.
|
