| Project/Area Number |
10671764
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
病態科学系歯学(含放射線系歯学)
|
|---|
| Research Institution | Tohoku Fukushi University |
|---|
Principal Investigator |
ABE Kazuhiko Tohoku Fukushi Univ., Associate Professor, 総合福祉学部, 助教授 (40151089)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ABBE Shoko (TAKAHASHI Shoko / TAKAHASHI-ABBE Shoko) Tohoku Univ. Graduate Sch., Sch. of Dentistry, Assistant Professor, 大学院・歯学研究科, 助手 (30175905)
|
|---|
| Project Period (FY) |
1998 – 2000
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | nucleotide metabolism / Streptococcus sanguinis / Streptococcus gordonii / mutans streptococci / nucleotidase / ammonia production / deaminase / 菌体外ヌクレオチド / (d)CMP / (d)AMP / Streptococcus sanguis / シチジン1リン酸(CMP) / 口腔細菌 / 嫌気代謝 / Streptococcus mutans / 口腔レンサ球菌 / 5'-ヌクレオチダーゼ / シチジンデアミナーゼ |
|---|
| Research Abstract |
It has been reported that various bacteria inhabit in an oral cavity and some of these bacteria may cause several diseases in the other area. Especially Streptococcus sanguinis has been detected in the inflammation part of endocarditis and in various abscess regions. In this study we examined whether oral streptococci metabolize extra-cellular nucleotide which is supposed to exist in abscess regions. As a result, it was confirmed that Streptococcus sanguinis, Streptococcus gordonii and Streptococcus salivarius metabolized CMP and produced the ammonia and inorganic phosphate. Therefore, we examined the metabolic difference both these two streptococcal groups in detail. Both bacteria group had significant degree of cytidine deaminase activity in the cell-free extracts. Although S. sanguinis, S. gordonii and S. salivarius contained membrane-bound 5'-nucleotidase activity, but mutans streptococci did not contain the activity. The 5'-nucleotidase activity in the cell-free extract of S. sanguinis fell off significantly by the oxygen flash. Certain kind of active oxygen may be generated and inactivate the 5'-nucleotidase. AMP, GMP, UMP, deoxyAMP, deoxyCMP, deoxyGMP were decomposed by S sanguinis cells and inorganic phosphate was produced. And it was confirmed that S. sanguinis cells generated ammonia from (deoxy-)AMP, (deoxy-)CMP. In this study we found that S. sanguinis, S. gordonii and S. salivarius could metabolize extra-cellular 5'-(deoxy) ribonucleotide. It was considered that these facts are important to understand the ecosystem of the bacteria in various regions of the body as well as the dental plaque.
|
