| Project/Area Number |
10671767
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | Ohu University (1999)
Hiroshima University (1998) |
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Principal Investigator |
ITO Hiroshi Ohu Univ., Sch. Of Dent,. Assistant Professor, 歯学部, 講師 (20184682)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIURA Junko Ohu Univ., Sch. Of Dent., Research Associate, 歯学部, 助手 (80316471)
二階 宏昌 広島大学, 歯学部, 教授 (60028735)
宮内 睦美 広島大学, 歯学部, 助手 (50169265)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | oral cancer / rat / 4NQ0 / palate / bone resorption / 舌癌 |
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| Research Abstract |
(1) For the purpose of clarifying genome abnormality of oral cancer, we administrated 4-nitroquinoline-1-oxide (4NQO) as drink water for twenty-nine F344 male rats in order to establish oral cancers. As a result, tongue cancer was found for 41 % of the rats, palate cancer was found for 68 % of the rats . The report to have seen the occurrence of the palate cancer to the high rate like the above in prescribing 4NQ0 as drink water seems not published so far.
(2) A cell culture derived from rat tongue cancer was established.
(3) In the surface of the bone which is situated on the neighborhood of the rat palate cancer tissue, osteoclasts were always seen. The experiment model in which the bone absorption by the oral cancer tissue occur seems to have been established.
(4) When localization of gultathione S-transferase P (GST-P) in tongue cancer tissue and palate cancer tissue was examined with immunohistochemistry, it was observed that the immunoreactivity of GST-P was lower in palate than in tounge.
(5) We investigated the localization of abnormal p53 protein in the rat tongue cancer and the palate cancer with immunohistchemistry, abnormal p53 protein was found only for just the small number cancer tissue.
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