The role of chemokine apical periodontitis

Research Project

Project/Area Number	10671771
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	病態科学系歯学(含放射線系歯学)
Research Institution	KYUSHU UNIVERSITY
Principal Investigator	NAGATA Kengo KYUSHU UNIVERSITY Faculty of Dental Science, Ass. Prof., 大学院・歯学研究院, 助手 (90189134)
Co-Investigator(Kenkyū-buntansha)	KABASHIMA Hiroaki KYUSHU UNIVERSITY Faculty of Dental Science, Ass. Prof., 大学院・歯学研究院, 助手 (20214504)
Project Period (FY)	1998 – 2000
Project Status	Completed (Fiscal Year 2000)
Budget Amount *help	¥3,100,000 (Direct Cost: ¥3,100,000) Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000) Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000) Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywords	Apical periodontitis / Chemokine
Research Abstract	To study the role of chemokine in the local inflammation, such as apical peridontitis, we examined the samples from patients with periapical granuloma by means of immunohistochemistry. IFN-γ producing protein-10 (IP-10) -producing cells, CXC-R3-positive cells and CCR3-positive cells could be detected in the samples of periapical granuloma. The ratio of the number of CXC-R3-positive cells to the number of infiltrated mononuclear cells was higher than that of CCR3-positive cells. As previous report we could detect IFN-γ-positive cells, but not IL-4-positive cells, in the samples of periapical granuloma. These findings suggest that IP-10-producing cells and CXC-R3-positive cells may be important in the selective infiltaration of Th1 subsets in the periapical granulation tissues. To study the process of bone regeneration we examined three samples of periapical regenerative tissue obtained from two patients under a guided tissue regeneration treatment in endodontic surgery by the immunohistochemical and enzyme histochemical methods. The regenerative tissue consisted of a large number of fibroblast-like cells and a small number of mononuclear cells. Fibroblast-like cells stained positively for alkaline phosphatase and osteopontin, whereas mononuclear cells stained positively for CD4. Interleukin-4-producing cells could be detected in adjacent sections. However, interferon-γ-producing cells could not be detected. These findings suggest that interleukin-4-producing cells may be one of the members associated with success in the human bone regeneration process in vivo.