| Project/Area Number |
10671777
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | Showa University |
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Principal Investigator |
HACHISU Reiko Showa University, School of Dentistry, Lecturer, 歯学部, 助手 (10164857)
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| Co-Investigator(Kenkyū-buntansha) |
HANAZAWA Tomomi Showa University, School of Dentistry, Lecturer, 歯学部, 助手 (20245872)
YAMAGUCHI Akira Nagasaki University, School of Dentistry, Professor, 歯学部, 教授 (00142430)
OKANO Tomohiro Showa University, School of Dentistry, Professor, 歯学部, 教授 (20124688)
田谷 あつ子 昭和大学, 歯学部, 助手 (00291675)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Radiation effects / bone formation / osteoblasts / cell differentiation / bone morphogenetic protein / 細胞の分化 / ラット / アルカリフォスファターゼ / 石灰化 |
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| Research Abstract |
Previous study showed that irradiation to rat osteoblast-like cells (ROB cells) retards growth and differentiation of the cells. In the osteoblast differentiation, bone-morphogenetic protein-2 (BMP-2) commits undifferentiated cells to osteo-chondrogenic lineage cells and promotes osteoblast maturation. In case of C2C12 myoblasts, addition of BMP-2 into the cell culture convert cell differentiation into osteoblasts. To assess the effects of BMP-2 on irradiated bone tissues, we examine the effects of BMP-2 in irradiated culture cells to induce osteoblast differentiation.
In ROB cells, irradiation with 4, 8, 12 Gy decreased the number of calcified bone nodules formed on culture day 12. Addition of BMP-2 into the irradiated ROB cells with 4 and 8 Gy partially recovered the formation of bone-like nodules. In C2C12 cells, irradiation with 4 and 8 Gy decreased the number of myotybe formed in culture. With the addition of BMP-2, irradiation with 4 and 8 Gy did not affect the induction of osteoblastic phenotypes such as induction of ALP activity and production of osteocalcin in C2C12 cells.
These results suggest that the addition of BMP-2 into irradiated cells recover the osteoblast differentiation from radiation damage and induce cell differentiation into osteoblasts from myoblasts. BMP-2 may be available to recover the bone formation after radiation injury. Further study is necessary to evaluat the effects of BMP-2 on irradiated tissues in vivo.
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