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Helicobacter pylori Infection and its Immune Response in Patients with Intraoral Tumors

Research Project

Project/Area Number 10671778
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

EBIHARA Yoko  TOKYO DENT.COLL.,DEPT.,PROFESSOR, 歯学部, 教授 (00129355)

Co-Investigator(Kenkyū-buntansha) OKUDA Katsuji  TOKYO DENT.COLL.,DEPT.DENTISTRY,PROFESSOR, 歯学部, 教授 (40085741)
ISHIHARA Kazuyuki  TOKYO DENT.COLL.,DEPT.DENTISTRY,LECTURER, 歯学部, 講師 (00212910)
MIURA Tadashi  TOKYO DENT.COLL.,DEPT.DENTISTRY,ASSISTANT, 歯学部, 助手 (10266570)
NOMA Hiroyasu  TOKYO DENT.COLL.,DEPT.DENTISTRY,PROFESSOR, 歯学部, 教授 (40085791)
水野 嘉夫  東京歯科大学, 歯学部, 教授 (40051750)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordshelicobacter pylori / oral cancer / transient bacteria
Research Abstract

We detected Helicobacter pylori (IL pylori) by the PCR method from the intraoral palaque, tongue, and saliva in patients with inraoral cancer and established a detection method. IL pylori was found at the gastic mucosa in 21 of 53 patients with intraoral cancer (36%). In the group of patients positive for H. pylori in the stomach, intraoral H. pylori was detected at a rate as high as 52% (11 patients). When the antagonism of intraoral normal bacteria flora to H. pylori was investigated, H. pylori inhibited the growth of most of the bacteria flora. When we looked at cocoagulation with H. pylori the organism was coagulated with only Fusobacterium nucleatum and porphyromonas gingivalis, but not other bacteria. In separate experiments, DNA amplified by PCR was cut by restriction enzymes such as Hae III, Ras I, Hiu fi, or Tag I in order to investigate its multiformation. This confirmed that both IL. Pylori in the mouth and gastric mucosa were genetically identical.
Although the presence of H. pylori in the mouth is clear from this finding, condition leading to the migration of H. pylori to the mouth and their growth there are poor and it was thus assumed that H. pylori might be present in there only temporarily by gastric reflux. However, in patients with intraoral cancer in whom the immunecompetence is decreased, while some H. pylori may be eliminated by the antagonism of intraoral normal bacteria flora, but remaining H. pylori may be trapped by other bacteria and thus be present at a high rate in the mouth. H. pylori was detected from the mucosa on the surface of intraoral cancer in three of 11 patients in whom H. pylori was found in the mouth. The bacteria were localized in the marginal epithelium of the cancer lesion, which was shown by postoperative H. E. staining. However, the number of the bacteria was so small that their direct involvement in the development of intraoral cancer was considered unlikely.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 奥田克爾: "歯周治療はピロリ菌感染消化性潰瘍の治療に不可欠か"The Nippon Dental Review. 665. 139-148 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katuji Okuda: "Relationships between chronic oral infectious diseases and systemic diseases"The bulletin of Tokyo Dental College. 39. 165-174 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katuji Okuda: "Helicobacter pylori May Have Only a Transient in the Oral Cavity and the Surface of Oral Canncer"Microbiology and Immunology. 44(5)(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katuji Okuda: "Necessity for treatment of Helicobacter pylori in Periodentitis"The Nippon Dental Review. 665. 139-148 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katuji Okuda: "Relationships between chronic oral infetious diseases and systemic diseases"The bulletin of Tokyo Dental College. 39. 165-174 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katuji Okuda: "Helicobacter pylori May Have Only a Transient Presence in the Oral Cavity and the Surface of Oral Cancer"Microbiology and Immunology. 44(5)(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katuji Okuda: "Helicobacter pylori May Have Only a Transient Presence in the Oral Cavity and the Surface of Oral Cancer"Microbiology and Immunology. 44(5)(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kazuyuki Ishihara: "Oral bacteria inhibit Helicobacter pylori growth" FEMS Microbiology Letters. 152. 355-361 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 奥田 克爾: "歯周治療はピロリ菌感染消化性潰瘍の治療に不可欠か" The Nippon Dental Review. 665. 139-148 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Katuji okuda: "Relationships between chronic oral infectious diseases and systemic diseases" The bulletin of Tokyo Dental College. 39. 165-174 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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