| Project/Area Number |
10671786
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | National Institute of Radiological Science |
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Principal Investigator |
MAJIMA Hideyuki National Institute of Radiological Sciences, International Space Radiation Laboratory, Researcher, 国際宇宙放射線医学研究センター, 研究員 (60165701)
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| Co-Investigator(Kenkyū-buntansha) |
ANDO Koichi National Institute of Radiological Sciences, International Space Radiation Laboratory, Researcher, 国際宇宙放射線医学研究センター, 研究員 (00159526)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Radiation / Apoptosis / Mitochondria / Mechanisms of Cell Death / Free Radical / Sensitivity / Vitamin E / 染色体異常 / 生存率 / DNA2重鎖切断 |
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| Research Abstract |
Mitochondrial DNA codes 13 components of electron transport chain. Lacking of mtDNA abrogates the electron transport system. We hypothesize that mitochondria deficient in functional electron transport system can result in increased production of superoxide and thus more oxidative stress. Sensitivity to X-rays, clonogenicity, frequency of double strand breaks(DSB), chromosome aberration, free radical generation, apoptosis and oxidative damage markers were examined in 143B human oseteosarcoma cell line (Rho+) and mtDNA-less (Rho0) cells derived from this cell line. No significant difference was observed between Rho+ and Rho0 cells without X-rays exposure for frequency of DSB and chromosome aberration. Clonogenicity assay showed greater sensitivity in Rho0 cells compared to Rho+ cells exposed to X-rays. More DSBs, and more chromosome aberrations in terms of gap, translocation, and deletion were observed in Rho0 cells. Substitution of normal mitochondria including mitochondrial DNA resulted recover of X-ray sensitivity. These results suggest that Rho0 cells lacking mtDNA has more oxidative stress and higher sensitivity to X-rays. Furthermore, the chromosomal damage by irradiation was restored substantially by Vitamin E.
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