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A study on the influences of changes of regional tissue blood flow volume on energy metabolism in masticatory muscles

Research Project

Project/Area Number 10671811
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

YAMAGUCHI Taihiko  Hokkaido Univ., Dental Hospital, Lecturer, 歯学部・付属病院, 講師 (90200617)

Co-Investigator(Kenkyū-buntansha) KOMATSU Kosetsu  Hokkaido Univ., School of Dentistry, Instructor, 歯学部, 助手 (90271668)
INOUE Nobuo  Hokkaido Univ., Dental Hospital, Associate Prof., 歯学部・付属病院, 助教授 (20091415)
TOTSUYA Yasunori  Hokkaido Univ., School of Dentistry, Prof., 歯学部, 教授 (00109456)
SATOH Kaoru  Hokkaido Univ., Dental Hospital, Instructor, 歯学部・付属病院, 助手 (40281828)
MINOWA Kazuyuki  Hokkaido Univ., School of Dentistry, Instructor, 歯学部, 助手 (30209845)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsblood flow volume / masticatory muscles / energy metabolism / 31P-MRS
Research Abstract

In order to clarify the influences of chronic decrease in blood flow volume on energy metabolism in masticatory muscles, we tried to establish the method for obtaining 31P-spectra by using NMR, and tried to make the the wister rat model in which the blood flow volume of the gastrocneminus muscles and the soleus muscles chronically continued to decrease with bound dominant artery. As the parameters of regional tissue blood flow volume, oxygen saturation level of blood (StO2) and total hemoglobin (Hb) volume were measured using a three wavelength spectrophotometric method.
The results were as follows;
1. Using a 7T NMR and a surface coil (25mm diameter), we obtained the 31P-spectra of ATP sample, and we were able to detect peaks of α, β, γ-ATP. However, the 31P-spectra of rat muscles were not obtained clearly because of noise from surrounding tissues. This problem is expected to be solved by improvement in program of control system of the NMR.
2. We obtained precise reproduciblity in measurement of StO2 and Hb volume, when we fixed both the probe of measuring apparatus and the leg of rats using devices which we made for fixation appropriately.
3. Just after binding of the artery, the value of StO2 and total Hb volume declined by about 30% and 70%, respectively. These levels continued one or two months after binding. Therefore, it was suggested that we could make the relatively chronic model in which the blood flow volume of the gastrocneminus muscle and the soleus muscles decreased obviously.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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