| Project/Area Number |
10671889
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
ITOH Michiichiro (2000-2001) 1st Dep. of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, Assistant Professor, 歯学部・附属病院, 講師 (70223188)
空閑 祥浩 (1998-1999) 長崎大学, 歯学部, 助教授 (00047820)
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| Co-Investigator(Kenkyū-buntansha) |
FURUKAWA Koichi Dep. of Biochemistry II, Nagoya University School of Medicine, Professor, 医学部, 教授 (80211530)
福本 敏 長崎大学, 歯学部, 助手 (30264253)
伊藤 道一郎 長崎大学, 歯学部, 助手 (70223188)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Glycosphinaolipids / nerve regeneration / clinical application |
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| Research Abstract |
We previously reported that a gangliosides mixture from bovine brain could prevent neuronal death and promote regeneration in rats with hypoglossal nerve resection. In the present study, we have compared the neurotrophic effects of various glycosphingolipids including lactosyl-ceramide. "Methods" Wistar rats were anesthetized and a 5 mm segment of the right hypoglossal nerve was removed. In the gangliosides-injected group, 2μg gangliosides dissolved in PBS was injected into the nerve stump site. In the autografted group, the 5 mm section hypoglossal nerve was replaced back between the excised nerve stumps and sutured to the stump. Injection of synthetic gangliosides, neutral glycolipids, oligosaccarides and ceramides were also administered in asimilar manner.
The findings that GTlb had the activity of neuronal death prevention equivalent to a ganglioside mixture or autograft, while other glycolipids exhibited about 60 % activity. However, the capability to promote the regeneration varied among glycolipids, that is, GT1b (86 %), GD1b (55 %), GD1a (35 %), GQ1b (34 %), GM1 (20 %), lactosyl-ceramide (17 %) in the number of horseradish peroxidase-positive neurons as an indicator of regeneration. The experiments with oligosaccharides of GTlb or GDlb and ceramide showed that the carbohydrate moiety mainly exerts neurotrophic effects.
These findings suggested that fine structures of carbohydrate moiety in gangliosides are critical in the regenerative activity in this hypoglossal nerve regeneration system.
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