THE ESTABLISHMENT OF ORAL CANCER THERAPY USING TISSUE SPECIFIC INF-r GENE
| Project/Area Number |
10671906
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Showa University |
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Principal Investigator |
YAGAMI Kimitoshi (1999) Showa University, School of Dentistry, Lecturer, 歯学部, 講師 (00210211)
上條 竜太郎 (1998) 昭和大学, 歯学部, 講師 (70233939)
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| Co-Investigator(Kenkyū-buntansha) |
HATORI Masashi Showa University, School of Dentistry, Assistant Professor, 歯学部, 助手 (20245814)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | INF-r / Oral Cancer / B 16 melanoma / mouse / Invasion, metastasis / cytokine / B16メラノーマ細胞 / アデイウイルスベクター / 遺伝子導入 |
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| Research Abstract |
1. The establishment of transplanted and metastasized mouse with carcinoma cell line
We succeed establishment of the B 16 melanoma transplanted and metastasized mouse by using B 16 melanoma cells into C57/BL16 mouse subcutaneously and inter venously.
2. Transformation of IFN-r gene expression vector into B 16 melanoma
We succeed transformation of IFN-r gene expression vector (pAdex1CAmINF-r) into B 16 melanoma cells in culture. INF-r was detected from pAdex1CAmINF-r transformed B 16 melanoma culture medium.
The proliferation of the cells were not changed by the gene transformation. The production levels of INF-r were decreased after some passage.
3. Transformation of IFN-r gene expression vector into HSC-4 cells or human oral squamous cell carcinoma (NA)
We succeed transformation of IFN-r gene expression vector (pAdex1CAmINF-r) into HSC-4 cells or NA cells in culture. INF-r was detected from pAdex1CamINF-r transformed HSC-4 cells or NA cells culture medium.
4. Characterization of pAdex1CAmINF-r transformed HSC-4 cells or human oral squamous cell carcinoma (NA)
The proliferation of the cells were not changed by the gene transformation. The production levels of INF-r were decreased after some passage.
5. The establishment of transplanted and metastasized mouse with pAdex1CAmINF-r transformed HSC-4 cells or NA cells
Levels of the transformation and transplantation were very low. We did not detect IFN-r from transplanted tumors.
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Report
(3 results)
Research Products
(3 results)
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[Publications] Takizawa,K.,Kamijo,R.,Ito,D.,Sumitani,K.,Hatori,M.,Nagumo,M.: "Synergistic Induction of the ICAM-1 expression by cisplatin and 5-fluorouracil in a cancer cell line via the NF-kB independent pathway" British Journal of Cancer. (印刷中).
