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The effects of intravenous anesthetics on ischemia/reperfusion injury in rabbit heart

Research Project

Project/Area Number 10671913
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

YOSHIDA Kazuichi  Kanagawa Dental College, Anesthesiology, Asso.Prof., 歯学部, 助教授 (50200978)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Hisao  Kanagawa Dental College, Anesthesiology, Instructor, 歯学部, 助手 (20308315)
KANDA Motohiko  Kanagawa Dental College, Anesthesiology, Instructor, 歯学部, 助手 (40277900)
OHSAWA Akiyoshi  Kanagawa Dental College, Anesthesiology, Professor, 歯学部, 教授 (90084748)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Keywordsischemia / reperfusion / induced hypotension / intravenous anesthetics / myocardial protection / infarct size / nicardipine / nitroglycerine / ATP / cardioprotetion / propofol / thiopental / ketamine / verapamil / ischemic preconditioning / KATP channel / sevoflurane / xylazine / gllbenclamide
Research Abstract

This study was designed to elucidate comparative effects of intravenous anesthetics and induced hypotension with nitroglycerin, nicardipine, and ATP on the intensity of myocardial necrosis (infarct size) following ischemia and reperfusion.
Rabbits received regional ischemia by 30 min of the left anterior descending artery (LAD) occlusion followed by 3 hrs of reperfusion under ketamine/xylazine anesthesia. The animals were randomly assigned to a control group, propofol/ketamine and nicardipine, nitroglycerin or ATP treatment groups, in which a continuous infusion of nicardipine, nitroglycerin, or ATP was initiated 30 min prior to ischemia. The systolic arterial blood pressure was adjusted to about 80% of that in baseline and maintained throughout ischemia and reperfusion. The area at risk was delineated by Evans blue, and infarct size was determined by tetrazolium staining at the end of the experiment by re-ligation of LAD.
The area at risk showed no significant differences among three groups ; Infarct size/area at risk in the nicardipine and ATP rabbits was significantly reduced. However, intravenous anesthetics and nitroglycerin did not have an infarct size limiting effect, though volatile anesthetics such as sevoflurane and halothane have myocardial protective effect as compared with ketamine/xylazine anesthesia.
These results suggest that nicardipin, nitroglycerine did not worsen the intensity of myocardial necrosis and nicardipine and ATP have cardioprotective effects on the rabbit myocardium. Nitroglycerin, however, exerted less effects on heart rate and diastolic arterial blood pressure compared with nicardipine.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 古屋宗孝,吉田和市,大澤昭義: "低血圧麻酔下における心筋虚血再灌流障害の検討-ニカルジピン、ニトログリセリンの比較検討-"循環制御. 20(3). 281-287 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 丸山久雄,吉田和市,神田元彦,古屋宗孝,大澤昭義: "心筋虚血再灌流障害と麻酔薬による心筋保護効果-invivoウサギモデルにおける検討-."循環制御. 20(3). 288-293 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 古屋宗孝,吉田和市: "ベラパミルおよびニカルジピンが心筋虚血再灌流に及ぼす影響-in situウサギモデルにおける比較検討-"麻酔. 48(10). 1074-1082 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 吉田和市: "麻酔薬が心筋虚血再灌流に及ぼす影響"日本歯科麻酔学会雑誌. 27(3). 276-280 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Okubo,L.Xi,N.L.Bernardo K-I Yoshida Rakesh C.Kukreja: "Myocardial preconditioning : Basic concepts and potential mechanisms"Mol.& Cell.Bioch.. 196. 3-12 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 吉田和市,古屋宗孝,永井亨,大澤昭義: "ATPによる低血圧麻酔が心筋虚血再灌流障害に及ぼす影響"日本蘇生学会雑誌. 19(1). 23-27 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazu-ichi Yoshida, Akiyoshi Osawa: "Differential vascular sensitivity of canine mesenteric arteries and veins to sevoflurane"Journal of anesthesia. (1). 27-32 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirofumi Takano and Kazu-ichi Yoshida: "Myocardial ischemia and reperfusion arrhythmias and cardioprotection by lidocaine-A study in an in vivo rabbit model-"J.Jpn.Dent.Soc.Anesthesiol.. 26(2). 224-233 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Munetaka Furuya, Kazu-ichi Yoshida and Akiyoshi Ohwasa: "Comparative effects of induced hypoyension with nicardipine and nitroglycerin on myocardial ischemia and reperfusion injury"Circ Cont. 20. 281-287 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Munetaka Furuya, Kazu-ichi Yoshida: "Comparative effects of induced hypotension with nicardipine and nitroglycerine on myocardial ischemia and reperfusion injury"Circ Cont. 20(3). 281-287 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hisao Maruyama, Kazu-ichi Yoshida, Munetaka Furuya, Motohiko Kanda and Akiyoshi Ohsawa: "Efeects of volatile anesthetics on myocardial ischemia/reperfusion injury-A study in in-vivo rabbit model-"Circ Cont. 20(3). 288-293 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Munetaka Furuya, Kazu-ichi Yoshida: "Comparison of effects of verapamil and those of nicardipine on myocardial ischemia and reperfusion injury : A study in an in situ rabbit model"Masui. 48(10). 1074-1082 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazu-ichi Yoshida, Munetaka Furuya, Tohru Nagaiand Akiyoshi Ohsawa: "Effects of induced hypotension with ATP on myocardial ischemia and reperfusion injury."Jpn J Reanimatology. 19(1). 23-27 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazu-ichi Yoshida, Hirofumi Takano, Hideyuki Sawada, Munetaka Furuya, Akiyoshi Ohsawa: "Lidocaine protects the ischemic rabbit myocardium from infarction as well as ventricular arrhythmias"Am J anesthesiol.. 27(7). 419-423 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] kazu-ichi Yoshida: "Effects of anesthetic agent on myocardial ischemia/reperfusion injury"J Jpn Dent Soc Anesthesiol. 27(3). 276-280 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shinji Okubo, Lei Xi, Nelson L.Bernardo, Kazu-ichi Yoshida and Rakesh C.Kukreja: "Myocardial preconditioning : Basic concepts and potential mechanisms"Mol. & Cell.Bioch.. 196. 3-12 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 吉田和市,古屋宗孝,永井亨,大澤昭義: "ATPによる低血圧麻酔が心筋虚血再灌流障害に及ぼす影響"日本蘇生学会雑誌. 19(1). 23-27 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.-i.Yoshida,H.Takano,H Sawada,M.Furuya,A.Ohsawa: "Lidocaine protects the ischemic rabbit myocardium from infarction as well as ventricular arrhythmias"The American Journal of Anesthesiology. 27(7). 419-423 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 吉田和市: "麻酔薬が心筋虚血再灌流に及ぼす影響"日本歯科麻酔学会雑誌. 27・3. 276-280 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S Okubo, K-i Yoshida, et. al.: "Myocardial preconditioning: Basic concepts and potential mechanisms"Mol. & Cell. Bioch.. 196. 3-12 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 古屋宗孝、吉田和市ら: "低血圧麻酔下における心筋虚血再灌流障害の検討"循環制御. 20・3. 281-287 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 丸山久雄、吉田和市ら: "心筋虚血再灌流障害と麻酔薬による心筋保護効果"循環制御. 20・3. 288-293 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 古屋宗孝、吉田和市: "ベラパミルおよびニカルジピンが心筋虚血再灌流に及ぼす影響"麻酔. 48・10. 1074-1082 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 丸山久雄、吉田和市ら: "プロポフォール麻酔からの覚醒遅延の一症例"臨床麻酔. 23・8. 1355-1356 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 高野宏文、吉田和市: "心筋虚血再灌流不整脈とリドカインによる心筋保護効果" 日本歯科麻酔学会雑誌. 26(2). 224-233 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K-I.Yoshida,et al: "Intraoperative spasm of coronary and peripheral arteries" Anesthesia Progress. 45. 74-76 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Okubo,K-I. Yoshida,R.C.Kukreia,et al: "Myocardial preconditioning: Basic concepts and potential mechanisms" Mol.& Cell.Biochi. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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