| Project/Area Number |
10671913
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
YOSHIDA Kazuichi Kanagawa Dental College, Anesthesiology, Asso.Prof., 歯学部, 助教授 (50200978)
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| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Hisao Kanagawa Dental College, Anesthesiology, Instructor, 歯学部, 助手 (20308315)
KANDA Motohiko Kanagawa Dental College, Anesthesiology, Instructor, 歯学部, 助手 (40277900)
OHSAWA Akiyoshi Kanagawa Dental College, Anesthesiology, Professor, 歯学部, 教授 (90084748)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | ischemia / reperfusion / induced hypotension / intravenous anesthetics / myocardial protection / infarct size / nicardipine / nitroglycerine / ATP / cardioprotetion / propofol / thiopental / ketamine / verapamil / ischemic preconditioning / KATP channel / sevoflurane / xylazine / gllbenclamide |
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| Research Abstract |
This study was designed to elucidate comparative effects of intravenous anesthetics and induced hypotension with nitroglycerin, nicardipine, and ATP on the intensity of myocardial necrosis (infarct size) following ischemia and reperfusion.
Rabbits received regional ischemia by 30 min of the left anterior descending artery (LAD) occlusion followed by 3 hrs of reperfusion under ketamine/xylazine anesthesia. The animals were randomly assigned to a control group, propofol/ketamine and nicardipine, nitroglycerin or ATP treatment groups, in which a continuous infusion of nicardipine, nitroglycerin, or ATP was initiated 30 min prior to ischemia. The systolic arterial blood pressure was adjusted to about 80% of that in baseline and maintained throughout ischemia and reperfusion. The area at risk was delineated by Evans blue, and infarct size was determined by tetrazolium staining at the end of the experiment by re-ligation of LAD.
The area at risk showed no significant differences among three groups ; Infarct size/area at risk in the nicardipine and ATP rabbits was significantly reduced. However, intravenous anesthetics and nitroglycerin did not have an infarct size limiting effect, though volatile anesthetics such as sevoflurane and halothane have myocardial protective effect as compared with ketamine/xylazine anesthesia.
These results suggest that nicardipin, nitroglycerine did not worsen the intensity of myocardial necrosis and nicardipine and ATP have cardioprotective effects on the rabbit myocardium. Nitroglycerin, however, exerted less effects on heart rate and diastolic arterial blood pressure compared with nicardipine.
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