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Specific Periodontopathic Bacteria and Salivary Components in Individuals with Down syndrome

Research Project

Project/Area Number 10671934
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionOsaka University

Principal Investigator

AMANO Atsuo,dds,phD  Osaka University Dental Hospital, Assistant Professor, 歯学部・附属病院, 講師 (50193024)

Co-Investigator(Kenkyū-buntansha) MORI Yuko  Osaka University Dental Hospital, Instructor, 歯学部・附属病院, 医員
KISHIMA Tetsuhiko  Osaka University Dental Hospital, Instructor, 歯学部・附属病院, 医員
AKIYAMA Shigehisa  Osaka University Dental Hospital, Senior Instructor, 歯学部・附属病院, 助手 (00283797)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsDown syndrome / Early-onset periodontitis / PCR / Porphyromonas gingivalis / Bacterial prevalence / fimA / Genotypes / Periodontopathic bacteria / 歯周病 / porphyromonas gingivalis / fimA 遺伝子型 / 歯周病原性細菌 / Down Syndrome / Actinobaullus actinomycetemcomitans / Periodontitis
Research Abstract

It is widely known that individuals with Down syndrome (DS) often develop severe periodontal diseases. Although Actinobacillus actinomycetemcomitans is suspected to be a causative pathogen, it is still unclear which pathogens are predominantly involved. In this study, we surveyed the periodontal pathogens harboring in DS population to determine if specific pathogens are involved in the etiology of the periodontal diseases.
The subjects were 120 children including 60 DS children (2-13 years old, 5 subjects in each age bracket) and 60 agematched controls, and 62 DS adults (14-57 years old). Ten pathogens, Porphyromonas gingivalis, A. actinomycetemcomitans, Bacteroides forsythus, Treponema denticola, Prevotella intermedia, P. nigrescens, Capnocytophaga ochracea, C. sputigena, Campyrobacter rectus, and Eikonella corrodens were surveyed in subgingival plaque samples using a polymerase chain reaction. Periodontal status was evaluated by pocket probing depth, bleeding on probing, and gingival … More index.
No significant difference in periodontal status was observed between the DS and control groups, however, all of the pathogens were detected with greater frequency in the DS children. B. forsythus, T. denticola, P. nigrescens, and C. rectus were significantly prevalent throughout all age brackets of the DS children (p<0.01 or 0.05). The occurrence of P. gingvalis was also significant in the DS subjects over 8 years old. A cluster analysis of the microbial profiles of the DS subjects showed that gingivitis was associated with increased varieties of the harboring pathogens and the distribution of P. gingivalis.
The bacterial occurrences were analyzed in 62 DS adults (14-57 years old) with healthy or diseased periodontal status. The prevalence of P. gingivalis showed the relationship to severity of periodontitis in 14-35 yeas old DS subjects. Whereas, a majority of the older DS subjects (36-57 yeas old) showed advance periodontal destruction despite of bacterial profiles. To clarify the prevalence of specific genptypes of this organism, the distribition of four genotypes of fimA gene of P. gingivalis was further investigated in the subjects. P. gingivalis possessing type II or type Ib fimA gene was found to be significantly predominant.
These results suggest that colonization by various periodontal pathogens can occur in quite early childhood of the DS population. It is also suggested that P. gingivalis with specific genotypes promote the disease progression in DS adults. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Amauo A, Nakagawa I, Morisaki I et al.: "Distribution of Porphyromonas gingivalis strains with FunA genotypes in periodontitis patients"Journal of Clinical Microbiology. 37・5. 1426-1430 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 貴島徹彦、天野敦雄 他: "ダウン症候群児のpolymerase chain reaction法による歯肉縁下プラーク歯周病原菌の検出"障害者歯科. 20・1. 31-37 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amauo A, Nakamura T, Morisaki I et al.: "Molecular interactions of Porphyromonas gingivalis fimbriae with host proteins : kinetic analyses based on surface plasmon resonance"67・5. 2399-2405 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakamura T, Amauo A, Nakagawa I et al.: "Specific interactions between Porphyromonas gingivalis fimbriae and human extracellular matrix proteins"FEMS Microbiology Letters. 175. 267-272 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kataoka K, Amauo A, Kawabata et al.: "Secretion of functional salivary peptide by Streptococcus gordonii which inhibits fmbriae-mediated adhesion of Porphyromonas gingivalis"Infection and Immunity. 67・8. 3780-3785 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amano A, Kishima T et al.: "Periodontopathic bacteria harboring in children with Down syndrome"Journal of Periodontology. 71・2. 250-256 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 天野敦雄(分担): "障害者歯科ガイドブック5章「障害者の歯科治療:歯周治療」"医歯薬出版. 9 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amauo A, Nakagawa I, Hamada S(分担): ""Biofilms" In Methods in Enzymology"Academic Press. 13 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amano A, Shizukuishi S, Horie H, Kimura S, Morisaki I, Hamada S: "Binding of Porphyromonas gingivalis fimbriae to proline-rich glycoproteins in parotid saliva via a domain shared by major salivary components."Infection and Immunity. 66(5). 2072-2077 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kuboniwa M, Amano A, Shizukuishi S: "Hemoglobin-binding protein purified from Porhyromonas gingivalis is identical to lysine-specific cysteine proteinase (Lys-Gingipain)."Biochemical and Biophysical Research Communications. 249. 38-43 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amano A, Nakagawa I, Kataoka K, Morisaki I, Hamada S: "Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients."Journal of Clinical Microbiology. 37(5). 1426-1430 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amano A, Nakamura T, Kimura S, Morisaki I, Nakagawa I, Kawabata S, Hamada S: "Molecular interactions of Porphyromonas gingivalis fimbriae with host Proteins : kinetic analyses based on surface plasmon resonance."Infection and Immunity. 67(5). 2399-2405 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakamura T, Amano A, Nakagawa I, Hamada S: "Specific interactions between Porphyromonas gingivalis fimbriae and human extracellular matrix proteins."FEMS Microbiology Letters. 175. 267-272 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kataoka K, Amano A, Kawabata S, Nagata H, Hamada S, Shizukuishi S: "Secretion of functional salivary peptide by Streptococcus gordonii which inhibits fmbriae-mediated adhesion of Porphyromonas gingivalis."Infection and Immunity. 67(8). 3780-3785 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kontani M, Amano A, Nakamura T, Nakagawa I, Kawabata S, Hamada S: "Inhibitory effects of protamines on proteolytic and adhesive activities of Porphyromonas gingivalis."Infection and Immunity. 67(9). 4917-4920 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amano A, Kishima T, Kimura S, Takiguchi M, Ooshima T, Hamada S, Morisaki I: "Periodontopathic bacteria harboring in children with Down syndrome."Journal of Periodontology. 71(2). 250-256 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morisaki I, Fukui N, Fujimori Y, Murakami J, Daikoku H, Amano A: "Effects of combined oral treatment with cyclosporin A, nifedipine or diltiazem on drug-induced gingival overgrowth in rats."Journal of Periodontology. 71(3) (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mori Y, Amano A, Akiyama S, Morisaki I: "Effects of short professional mechanical tooth cleaning (PMTC) program in young adults with mental disabilities."Special Care in Dentistry. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakagawa I, Amano A, Kimura RK, Nakamura T, Kawabata S, Hamada S: "Distribution and molecular characterization of Porphyromonas gingivalis carrying a new type of fimA gene."Journal of Clinical Microbiology. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Daikoku H, Amano A, Fukui N, Akiyama S, Morisaki S: "Clinical evaluation of orofacial regulation therapy for Down syndrome children using Castillo-Morales palatal plate."Pediatric Dental Journal. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Amano A.,Nakagawa I.,Morisaki I.et al.: "Distribution of Porphyromonas gingivalis strains with funA genotypes in periodontitis patients"Journal of Clinical Microbiology. 37・5. 1426-1430 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 貴島徹彦,天野敦雄 他: "ダウン症候群児の polymerase chain reaction 法による歯肉縁下プラーク歯周病原菌の検出"障害者歯科. 20・1. 31-37 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Amano A.,Nakamura I.,Morisaki I.Et al.: "Molecular interactions of Porphyromonas gingivalis fimbriae with host proteins: kinetic analyses based on surface plasmon resonance"Infection and Immunity. 67・5. 2399-2405 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakamura T.,Amano A.,Nakagawa I.et al.: "Specific interactions between Porphyromonas gingivalis fimbriae and human extracellular matrix proteins"FEMS Microbiology Letters. 175. 267-272 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kataoka K.,Amano A.,Kawabata S.et al.: "Secretion of functional salivary peptide by Streptococcus gordonii which inhibits fmbriac-mediated adhesion of Porphyromonas gingivalis"Infection and Immunity. 67・8. 3780-3785 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Amano A.,Kishima T.et al.: "Periodontopathic bacteria harboring in children with Down syndrome"Journal of Periodontology. 71・2. 250-256 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 天野敦雄(分担): "障害者歯科ガイドブック5章 「障害者の歯科治療:歯周治療」"医歯薬出版. 285 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Amano A.,Nakagawa I.,Hamada S.(分担): ""Biofilms" In Mehods in Enzymology"Academic Press. 720 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 貴島徹彦・天野敦雄 他: "ダウン症候群児のpolymerase chain reaction法による歯肉縁下プラーク歯周病原性菌の検出" 障害者歯科. 20(印刷中). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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