| Project/Area Number |
10671970
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
HARA Yoshitaka Nagasaki University, School of Dentistry, Associate Professor, 歯学部, 助教授 (60159100)
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| Co-Investigator(Kenkyū-buntansha) |
UKAI Takashi Nagasaki University, School of Dentistry, Instructor, 歯学部, 助手 (20295091)
ABE Yoshihiro Nagasaki University, School of Dentistry, Instructor, 歯学部, 助手 (70202687)
KATO Ihachi Nagasaki University, School of Dentistry, Professor, 歯学部, 教授 (30005087)
KANEKO Takashi Nagasaki University, School of Dentistry, Instructor, 歯学部, 助手 (10284697)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Root resorption / Periodontal ligament tissue / Odontoclast / Histopathological study / COX2 / 歯根膜細胞 / 歯周組織再生 |
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| Research Abstract |
The present study examines whether periodontal ligament (PDL) tissue is capable of inducing root resorption. The distal root of the rat molar was sectioned with disk at the furcation and the PDL tissue was surgically removed from the root (non-PDL group, n=40). The distal root with the PDL intact was also prepared (PDL-intact group, n=40). The roots were transplanted into the dorsal skin of the rat. On the 1st, 3rd, 5th, 7th, 10th, 14th, 21st or 28th day after transplantation, the roots were removed together with surrounding dorsal subcutaneous tissue and were fixed, decalcified and embedded in paraffin. Serial sections from each block were stained with hematoxylin and eosin or tartrate-resistant acid phosphatase (TRAP) staining to observe odontoclast formation. Cyclooxygenase-2 (COX2) was also immunohistologically detected to observe prostaglandinEィイD22ィエD2 production. On the 7th day after transplantation, multinucleated odontoclasts with TRAP and root resorption were observed in the PDL-intact group. The number of odontoclasts peaked on the l0th day after transplantation. On the other hand, no odontoclastic root resorption was observed in the non-PDL group. COX2-positive cells were observed in PDL tissue during the early experimental stage. These results suggest that PDL tissue is involved in the formation of odontoclasts and root resorption.
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