• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Possible Role of Gingival Epithelial Cells in Host Defense Mechanism of Periodontal Disease Progression

Research Project

Project/Area Number 10671971
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionKagoshima University

Principal Investigator

IZUMI Yuichi  Kagoshima University, Faculty of Dentistry, Professor, 歯学部, 教授 (60159803)

Co-Investigator(Kenkyū-buntansha) MINAMI Mutsumi  Kagoshima University, Dental Hospital, Research Associate, 歯学部・附属病院, 助手 (60229771)
MATSUYAMA Takashi  Kagoshima University, Faculty of Dentistry, Research Associate, 歯学部, 助手 (40253900)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsPeriodontal disease / Gingival epithelial cells / Gingival crevicular fluid / Host defence mechanism / SLPI / IL-1 / IL-1ra / 分泌型白血球蛋白分解酵素阻害物質 / 生体防御
Research Abstract

The purpose of the present research project was to detect the expression of secretory leukocyte protease inhibitor (SLPI) and IL-1 receptor antagonist (IL-1ra) in gingival epithelial cells and to clarify the possible relationship between SLPI in gingival crevicular fluid (GCF) and the periodontal clinical parameters.
SLPI was immunocytochemically detected on frozen-sectioned gingival epithelium and expressed on the surface and inside of cultured gingival keratinocytes from healthy gingiva. The levels of SLPI and α1-protease inhibitor (α1-PI) and neutrophil elastase (NE) activity in GCF were measured and periodontal clinical parameters were examined in fifteen adult periodontitis patients. In thirty examined sites, significantly higher amounts of SLPI, α1-PI, NE and GCF were consistently obtained in samples from periodontitis sites than in samples from healthy sites. SLPI, α1-PI and NE were found to correlate with probing depth. NE andα1-PI were significantly correlated with increasing c … More linical attachment loss. These results suggested that SLPI andα1-PI may be related to the inflammation in periodontitis sites.
To clarify the expression of IL-1 and IL-1ra in human gingival keratinocytes, we studied the protein production and mRNA expression of IL-1α and IL-1ra in human cultured gingival keratinocytes using enzyme-linked immunosorbent assay and reverse transcription polymerase chain reaction (RT-PCR). Furthermore, we studied immunohistochemically the presence of IL-1αand IL-1ra in the epithelium of healthy and inflamed gingiva. The level of cell-associated IL-1α was significantly increased under stimulation of TNF-α, while the level of IL- 1α in conditioned medium and the levels of IL-1ra protein were not influenced by TNF-α. IL-1α and intracellular IL-1ra mRNA transcripts were enhanced under stimulation with TNF-αby RT-PCR. Furthermore, the presence of IL-1ra, but not IL-1α, found by immunohistochemistry in the epithelium of gingiva. These results indicated that human gingival keratinocytes expressed IL-1αand IL-1ra, and may be involved in the regulation of inflammation of periodontal disease by IL-1αand IL-1ra. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Takashi Hiraoka: "Immunochemical detection of CD14 on human gingival fibroblasts in vitro"Oral Microbiol Immunol. 13. 246-252 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 南 睦美: "成人性歯周炎患者の歯肉溝滲出液中のSecretory leukocyte protease inhibitor(SLPI)とα1-protease inhibitor(α1-PI)の検出"日歯周誌. 41. 28-35 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 楊 秋波: "ヒト歯肉角化細胞におけるInterleukin-1αおよびInterleukin-1receptor antagonistの発現について"日歯周誌. 41. 87-98 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuichi Izumi: "Effctive perodontal treatment in a patient with type IIA von Willebrand's disease I report of a case"J. Periodontol. 70. 548-553 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuichi Izumi: "Influence of different curette insertion depths on the outcome of non-surgical periodontal treatment"J. Clin. Periodontol. 26. 716-722 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takashi Matsuyama: "Expression and activity of thrombomodulin in human gingival epithelium: in vivo and in vitro study"J. Periodont. Res.. 35(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 和泉雄一: "歯周病における歯肉上皮細胞のはたらき"鹿歯紀. 20(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 伊藤学而: "臨床家のための矯正Year Book'98"クイレテッセンス出版. 291 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takashi Hiraoka: "Immunochemical detection of CD14 on human gingival fibroblasts in vitro"Oral Microbiol. Immunol.. 13. 246-252 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mutsumi Minami: "The levels of secretory leukocyte protease inhibitor and ∂1-protease inhibitor in gingival crevicular fluid from adult periodontal patients."J. Jpn. Soc. Periodontol. 41. 28-35 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Qiu-Bo Yang: "Expression of interleukin- 1∂ and interleukin-1 receptor antagonist in human gingival keratinocytes"J. Jpn. Soc. Periodontol. 41. 87-98 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuichi Izumi: "Effective periodontal treatment in patient with type IIA von Willebrand's disease: report of a case"J. Periodontol.. 70. 548-553 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuichi Izumi: "Influence of different curette insertion depths on the outcome of non-surgical periodontal treatment"J. Clin. Periodontol.. 26. 716-722 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takashi Matsuyama: "Expression and activity of thrombomodulin in human gingival epithelium: in vivo and in vitro study."J. Periodontol. Res.. 35. ((in press))

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuichi Izumi: "The role of gingival epithelial cells in periodontal disease progression"Ann. Kagoshima Dent.. 20. ((in press))

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 南 睦美: "成人性歯周炎患者の歯肉溝滲出液中のsecretory leukocyte protease inhibitor(SLPI)とα1-protease inhibitor(α1-PI)の検出"日歯周誌. 41・1. 28-35 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 楊 秋波: "ヒト歯肉角化細胞におけるInterleukin-1αおよびInterleukin-1 receptor antagonistの発現について"日歯周誌. 41・2. 87-98 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuichi Izumi: "Effective periodontal treatment in a patient with type IIA von Willebrand's disease of a case"J.Periodontal.. 70・5. 548-553 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuichi Izumi: "Influence of different curette insertion dephhs on the outcome of non-surgical periodontal treatment."J.Clin.Pereodontal.. 26・11. 716-722 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 和泉 雄一: "歯周病における歯肉上皮細胞のはたらき"鹿歯紀. 20(印刷中). (2000)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi