• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

PHARAMACEUTICAL STUEDIES ON GENE MATERIALS-CARRIER SYSTEM

Research Project

Project/Area Number 10672023
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionNagoya City University

Principal Investigator

YOTSUYANAGI Toshihisa (2001)  NAGOYA CITY UNIVERSITY, PHARMACEUTICAL SCIENCE, PROFESSOR, 薬学部, 教授 (06672150)

四ッ柳 智久 (四ツ柳 智久) (1998-2000)  名古屋市立大学, 薬学部, 教授 (40080189)

Co-Investigator(Kenkyū-buntansha) HAZEMOTO Norio  NAGOYA CITY UNIVERSITY, PHARMACEUTICAL SCIENCE, ASSOCIATE PROFESSOR, 薬学部, 助教授 (40192273)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsGENE DELIVERY / DENDRITIC PEPTIDE / LIPOSOME / POLYAMINO ACID / FREEZE-DRYING / TRANSFECTION EFFICIENCY / PLASMID DNA / SUGAR / MAP / DNA複合体 / 親和性 / 複合体の解離 / 粒子径 / 凝縮 / 塩基性polypeptide / カチオン性liposome / DNA / poly-L-lysine / liposome複合体 / DNA細胞内導入 / pSV2CAT / コレステロール誘導体
Research Abstract

The development of liposome and ionic polymer proceeds as gene carriers for the non-virus genetic treatment. In this research, we studied on a reagent consisting of liposome and peptides and on the freezing dry of the gene material. Liposome containing cholesterol derivative was used to improve the gene transfer efficiency of the basic polypeptide. Furthermore, dendritic peptide which had the branched structure was synthesized, and the genetic transfer efficiency under co-existence of liposome was examined. As an evaluation of gene expression, CAT activity (pSV2CAT) and luciferase activity (pGL3) in the HeLaS3 cell were assayed. The dendritic-peptide together with liposomes increased 2-3 times genetic expression compared with liposome only. The length of the side chain of dendritic peptide influenced gene expression. In four branched peptide, dendritic peptide of nine lysine chains accelated gene expression than three lysine chains. A remarkable difference was not observed in genetic e … More xpression between poly-L-lysine and dendritic peptide, though a optimal concentration of dendritic peptide in gene expression was shifted to the lower concentration compared with the case of poly-L-lysine. Complexes of plasmid DNA and polyamino acid showed high luciferase activity of the eight order of 10. Amounts of genetic expression decreased to about one-hundredths when complexes were freeze-dried and it dispersed again. Gene expression was recovered by addition of sugar in freeze-drying. The sugar effect in gene transfer was large in sucrose and trehalose. Effect on addition of the sugar in freezing dryness may be to aid the dispersion DNA complexes in freezing media, and to prevent the injury of the DNA molecule by a crystallization of water and to suppress a irreversible adhesion between DNA complexes at the time of dryness, and to suppress a irreversible adhesion between DNA complexes at the time of dryness. In this study, we characterized the DNA medicine manufactured by freezing dryness on biological aspect of the genetic expression as well as physicochemical aspect of complex dispersion. Less

Report

(4 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Norio Hazemoto: "Gene delivery mediated by synthetic dendritic peptide"Proceedings of the Conference on Frontiers Drug Development. 57-58 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 赤毛義美: "Cationic Liposomeを用いた癌に対する遺伝子導入の基礎的検討"Biotherapy. 12・1. 199-201 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 四ツ柳智久: "カチオン性リポソームをベクターとする細胞内遺伝子導入法"日本臨床. 56・3. 153-160 (1998)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi