The reaction mechanism of the ribonuclease T2 family

• Project/Area Number: 10672025
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Showa University
• Principal Investigator: NAKAMURA Kazuo Showa University, School of Pharmaceutical Sciences, Professor, 薬学部, 教授 (00012675)
• Project Period (FY): 1998 – 2001
• Project Status: Completed (Fiscal Year 2001)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: ribonuclease / X-ray structure determination / substrate analogue / subsite / リボ核酸分解酵素

Research Abstract

The RNase T2 family shows broad base specificity. The objects of the project on the RNase T2 family are to gain the base-recognition and catalytic mechanism of the RNase T2 family enzymes. The substrate-fragment complexs of RNase LE reveal interesting features : the substrate fragments (3'-GMP and 5'-GMP) and substrate analogue d(GpG) bound in the productive binding mode while inhibitor (2'-GMP) bound in the non-productive binding mode ; and the binding mode of 3'-GMP at the Bi subsite and that of 5'-GMP at the B2 site are similar to that of d(GpG) bound at both B 1 and B2 subsite. These observations show that the binding of substrate fragments may conform to the principles of additivity.

Research Products

• [Publications] Nobutada Tanaka, et al.: "Crystallization and preliminary X-ray crystallographic studies of Ribonuclease from Lycopersicon esculentum"Protein and Peptide Letters. 6・6. 407-410 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nobutada Tanaka, et al.: "Crystal Strucure of a Plant Ribonuclease, RNase LE"Journal of Molecular Biology. 298. 859-873 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tokumi Maruyama, et al.: "Synthesis of 2'-deoxy-2'-fluoroguanyl-(3',5')-guanosine"Nucleosides, Nucleotides and Nucleic Acids. (印刷中). (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 桐野豊, 中村和郎他: "生命薬学テキストシリーズ「物理化学(上)」"共立出版. 300 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] N. Tanaka, J. Aral, N. Inokuchi, T. Koyama, K. Ohgi, M. Irie, and K. T. Nakamura: "Crystallization and Preliminary X-ray Crystallographic Studies of Ribonuclease from Lycopersicon esculentum"Protein and Peptide Letters. 6. 407-410 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] N. Tanaka, J. Aral, N. Inokuchi, T. Koyama, K. Ohgi, M. Irie, and K. T. Nakamura: "Crystal Structure of a Plant Ribonuclease, RNase LE"J. Mol. Biol. 298. 859-873 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] T. Maruyama, S. Kozai, K. T. Nakamura, and M. Irie: "Synthesis of 2'-deoxy-2'-fluoroguanyl-(3', 5')-guanosine Nucleosides"Nucleotides and Nucleic Acids. (in press). (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hiroshi Uemura: "Crystallization and preliminary X-ray crystallographic studies of human autocrine motility factor"Protein and Peptide Letters. 8・4. 317-322 (2001)
• [Publications] Kiyoshi Ito: "The mechanism of substrate recognition of pyroglutamyl-peptidase I from Bacillus amyloliquefaciens as determined by X-ray Crystallography"Journal of Biological Chemistry. 276・21. 18557-18562 (2001)
• [Publications] Nobutada Tanaka: "Crystallization and Preliminary X-ray Crystallographic Studies of Ribonuclease LE from Lycopersicon Esculentum"Protein and Peptide Latters. 6・6. 407-410 (1999)
• [Publications] 桐野豊、中村和郎他: "生命薬学テキストシリーズ「物理化学(上)」" 共立出版, 約300頁 (1999)