| Project/Area Number |
10672035
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
ANZI Kazunori National Institute of Radiological Sciences, Bioregulation Research Group, Sub-group Leader, 第1研究グループ, 研究員 (70128643)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | MC-PROXYL / carbomoyl-PROXYL / autoradiography / 3D-ESR imaging / blood-brain-barrier / radio protection / rat brain / C3H mouse / methoxycarbonyl-PROXYL / 全身オートラジオグラフィー / 神経毒性 / ^<14>C標識スピンプローブ / 血液脳関門 |
|---|
| Research Abstract |
[ィイD114ィエD1C] carbamoyl-PROXYL and [ィイD114ィエD1C] methoxycarbonyl-PROXYL ([ィイD114ィエD1C] MC-PROXYL) were synthesized using liquid ammonia and [ィイD114ィエD1C] acetone as starting materials. The autoradiography of rats using these probes clearly demonstrated that [ィイD114ィエD1C] MC-PROXYL quickly distributed to brain through blood-brain-barrier whereas [ィイD114ィエD1C]carbamoyl-PROXYL did not. [ィイD114ィエD1C]MC-PROXYL distributed more to liver. Three-dimensional ESR images of the brain of rats taken with MC-PROXYL and carbamoyl-PROXYL suggested that MC-PROXYL is useful to make an image of rat brain. Radio protective effect of these spin probes was measured. MC-PROXYL injected to C3H mice (10 weeks old, male) at a dose of 600 mg/kg prolonged the survival of mice or even rescued (20% of total) the mice irradiated with γ-ray at 8.7 Gy. This finding suggests that MC-PROXYL may interact with free radicals generated by X-irradiation and lose the stable spin of the MC-PROXYL, which may be one of the mechanisms of spin clearance observed in in vivo ESR measurements.
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