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A Study on the Mechanism of Spin Clearance in In vivo ESR Measurement

Research Project

Project/Area Number 10672035
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionNational Institute of Radiological Sciences

Principal Investigator

ANZI Kazunori  National Institute of Radiological Sciences, Bioregulation Research Group, Sub-group Leader, 第1研究グループ, 研究員 (70128643)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsMC-PROXYL / carbomoyl-PROXYL / autoradiography / 3D-ESR imaging / blood-brain-barrier / radio protection / rat brain / C3H mouse / methoxycarbonyl-PROXYL / 全身オートラジオグラフィー / 神経毒性 / ^<14>C標識スピンプローブ / 血液脳関門
Research Abstract

[ィイD114ィエD1C] carbamoyl-PROXYL and [ィイD114ィエD1C] methoxycarbonyl-PROXYL ([ィイD114ィエD1C] MC-PROXYL) were synthesized using liquid ammonia and [ィイD114ィエD1C] acetone as starting materials. The autoradiography of rats using these probes clearly demonstrated that [ィイD114ィエD1C] MC-PROXYL quickly distributed to brain through blood-brain-barrier whereas [ィイD114ィエD1C]carbamoyl-PROXYL did not. [ィイD114ィエD1C]MC-PROXYL distributed more to liver. Three-dimensional ESR images of the brain of rats taken with MC-PROXYL and carbamoyl-PROXYL suggested that MC-PROXYL is useful to make an image of rat brain. Radio protective effect of these spin probes was measured. MC-PROXYL injected to C3H mice (10 weeks old, male) at a dose of 600 mg/kg prolonged the survival of mice or even rescued (20% of total) the mice irradiated with γ-ray at 8.7 Gy. This finding suggests that MC-PROXYL may interact with free radicals generated by X-irradiation and lose the stable spin of the MC-PROXYL, which may be one of the mechanisms of spin clearance observed in in vivo ESR measurements.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] K.Anzai 他5名: "Effects of hydroxyl radical and sulfhydryl radical・・・"Biochem. Biophys. Res. Commun.. 249. 938-942 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Anzai 他6名: "In vitro studies of the antioxidant activity of・・・"Res. Commun. Bichem. Cell & Mol. Biol.. 2. 129-138 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 安西和紀 他6名: "スピンプローブの脳への分布"磁気共鳴と医学. 10. 119-122 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Anzai 他5名: "Oxidation-dependent changes in the stability and・・・"Antioxidants & Redox Signaling. 1. 339-347 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Anzai 他3名: "Oxidative modification of ion channel activity・・・"Antioxidants & Redox Signaling. 2(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 安西和紀他7名: "Methoxycarbonyl-PROXYLを用いたラット脳の三次元ESRイメージング"磁気共鳴と医学. 11(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 安西和紀,三浦ゆり 他5名: "スピンプローブの脳への分布" 磁気共鳴と医学. 10. 119-122 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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