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Construction of cyborg lectins by using lambda foo phage display system, and structural analyses of the lectins complexed with sugars.

Research Project

Project/Area Number 10672038
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

YAMAMOTO Kazuo  The University of Tokyo, Graduate school of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (20174782)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordslectin / phage display system / lambda foo / Bauhinia purpurea lectin / λfooファージ / T7ファージ
Research Abstract

Bauhinia purpurea lectin (BPA) is one of β-galactose-binding leguminous lectins. Leguminous lectins contain a long metal-binding loop, the part of which determined of the carbohydrate-binding specificities. In the cDNA coding BPA, random mutations were introduced into a portion of cDNA which corresponds to the carbohydrate-binding loop of this lectin. Mutated lectin library expressed on the surface of lambda foo phages were screened by the panning method. Several phage clones having the affinity for mannose or N-acetylglucosamine were isolated, respectively. These results open new possibilities for making an artificial lectins (named "cyborg lectin") which have distinct and desired carbohydrate-binding specificities.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 山本一夫他2名: "Chimeric lectin of Bauhimia purpurea lectin and Lens culivaris lectin recognizes unique carbohydrate structure"J. Biochem.. 127(1). 129-135 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山本一夫他2名: "Cyborg lectins Novel leguminous lectins having unique specificities"J. Biochem.. 127(1). 129-135 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山本一夫他4名: "Order and maximum incorporation of N-acetylgalactosamine into threonine residues of MUC2 core peptide"J. Biochem.. 346(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Yamamoto, I. N. Maruyama, T. Osawa.: "Cyborg lectins : Novel leguminous lectins having unique specificities."J. Biochem.. 127. 137-142 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Yamamoto, Y. Konami, T. Osawa.: "Chimeric lectin of Bauhinia purpurea lectin and Lens culinaris lectin recognizes unique carbohydrate structure."J. Biochem.. 127. 129-135 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Torii, K. Yamane, T. Mashima, N. Haga, K. Yamamoto, J. W. Fox, M. Naito, T. Tsuruo.: "Molecular cloning and functional analysis of Apoxin I, a snake venom derived apoptosis-inducing factor with L-amino acid oxidase activity."Biochemisty. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Iida, H. Takeuchi, K. Kato, K. Yamamoto, T. Irimura.: "Order and maximum incorporation of N-acetylgalactosamine into threonine residues of MUC2 core peptide with microsome fraction of human colon carcinoma LS174T cells."Biochem. J.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Iida, K. Yamamoto, T. Irimura.: "Interaction of human macrophage C-type lectin with O-linked N-acetylgalactosamine residues on mucin glycopeptides."J. Biol. Chem.. 274. 10697-10705 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Konami, K. Yamamoto.: "Cyborg lectins : an approach for the construction of lectins having unique specificities."Glycoconj. J.. 16. S26 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Kurono, K. Yamamoto, T. Irimura, Y. Nagai.: "Attempts at qualitative and quantitative analysis of noncovalent and specifically bound complexes (protein and sugar chain) by the use of mass spectrometry."Glycoconj. J.. 16. S111 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Iimura, H. Ishikawa, K. Yamamoto and F. Sehnal.: "Hemagglutinating Properties of Apolipophorin III From the Hemolymph of Galleria mellonella Larvae."Arch. of Insect Biochem. and Physiol.. 38. 119-125 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Yamamoto, T. Tsuji, T. Osawa.: "Analysis of Asparagine-linked oligosaccharides by sequential lectin-affinity chromatography."Methods in Mol. Biol.. 76. 35-51 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山本一夫 他2名: "Chimeric lectin of Bauhinia purpurealectin and Lens culinaris lectin recognizes unique carbohydrate structure"J. Biochem.. 127(1). 129-135 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 山本一夫 他2名: "Cyborg lectins : Novel leguminous lectins having unique spacificities"J. Biochem.. 127(1). 137-142 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 山本一夫 他4名: "Order and maximum incorporation of N-acetylgalactosamine inte threonine residues of MUC2 core peptide"Biochem. J.. 346(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 山本一夫: "Analysis of Asparagine-linked oligosaccharides by sequential lectin-affinity chromatography" Methods in Mol.Biol.76. 35-51 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 飯田慎一郎: "Interaction of human macrophage C-type lectin with O-linked N-acetylgalactosamine residues on mucin glycopeptioes" J.Biol.Chem.274(印刷中). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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