Construction of cyborg lectins by using lambda foo phage display system, and structural analyses of the lectins complexed with sugars.
| Project/Area Number |
10672038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMAMOTO Kazuo The University of Tokyo, Graduate school of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (20174782)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | lectin / phage display system / lambda foo / Bauhinia purpurea lectin / λfooファージ / T7ファージ |
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| Research Abstract |
Bauhinia purpurea lectin (BPA) is one of β-galactose-binding leguminous lectins. Leguminous lectins contain a long metal-binding loop, the part of which determined of the carbohydrate-binding specificities. In the cDNA coding BPA, random mutations were introduced into a portion of cDNA which corresponds to the carbohydrate-binding loop of this lectin. Mutated lectin library expressed on the surface of lambda foo phages were screened by the panning method. Several phage clones having the affinity for mannose or N-acetylglucosamine were isolated, respectively. These results open new possibilities for making an artificial lectins (named "cyborg lectin") which have distinct and desired carbohydrate-binding specificities.
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Report
(3 results)
Research Products
(17 results)
