Analysis of Carrier-Mediated Transport Systems in Drug Absorption from Oral Mucosa
| Project/Area Number |
10672041
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
KIMURA Toshikiro Okayama University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (10025710)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWARA Ken-ichi Okayama University, Graduate School of Natural Science and Technology, Research Associate, 大学院・自然科学研究科, 助手 (30291470)
HIGAKI Kazutaka Okayama University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (60284080)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | human oral mucosal cells / D-glucose / dipeptide / cultured stratified cell layer / active transport / facilitated diffusion / transporter |
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| Research Abstract |
The in vitro uptake study was performed using the isolated cells of human oral mucosa, buccal and the dorsum of tongue, to investigate the mechanisms of glucose and oligopeptide uptake. The uptake of D-glucose was much larger in cells of the dorsum of tongue than in buccal cells and was inhibited more extensively by 2-deoxy-D-glucose, a substrate of facilitative glucose transporters, than by α-methyl-D-glucoside, a specific substrate of SGLT1, suggesting the larger contribution of facilitative transporter than NaィイD1+ィエD1/glucose cotransporter. Furthermore, from the results of inhibition studies by the several sugar analogues including maltose and D-mannose, GLUT1 and/or GLUT3 were suggested to take part in the glucose uptake by oral mucosa. Therefore, we have attempted to confirm the expression of glucose transporters on the oral mucosa by employing Western blotting. As a result, it was suggested that SGLT1, GLUT1, GLUT2 and GLUT3 are expressed in the epithelial cells of human oral mucosa. The result of the uptake study of glycylsarcosine, a model dipeptide, by isolated oral cells showed the presence of carrier-mediated transport systems for oligopeptides in human oral mucosal cells. The transport of D-glucose and glycylsarcosine across stratified cell layer of cultured human oral mucosal cells was also examined, and the results showed that the transporters function not only for the uptake of these substrates by the mucosal cells but also for the transport across the stratified mucosal cell layers.
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Report
(3 results)
Research Products
(3 results)
