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Molecular analysis and sensitivity to arachidonic acid of voltage-dependent KィイD1+ィエD1 channel, Kv4.3

Research Project

Project/Area Number 10672049
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionNAGOYA CITY UNIVERSITY

Principal Investigator

MAIZUMI Yuji  NAGOYA CITY UNIVERSITY, PHARMACOLOGY & THERAPEUTICS, PROFESSOR, 薬学部, 教授 (60117794)

Co-Investigator(Kenkyū-buntansha) OHYA Susumu  NAGOYA CITY UNIVERSITY, PHARMACOLOGY & THERAPEUTICS, JUNIOR LECTURER, 薬学部, 助手 (70275147)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsKv4.3 / in situ hybridization / RT-PCR / C-terminal mutagenesis / HEK293 cells / rate of recovery / hippocampus / ラット平滑筋 / 形質導入 / 電流キネティクス / 4-アミノピリジン
Research Abstract

In situ hybridization and RT-PCR analyses has revealed that, among three Kv4.3 splice variants (a, b, and c) with distinct C-terminal cytoplasmic domains, the mRNA for Kv4.3a is abundant in cerebral cortex, cerebellum, olfactory bulb, and medulla oblongata, whereas the mRNA of Kv4.3c is localized mainly to hippocampus. Three new distinct splice variants of Kv4.3 (Kv4.3d, e and f), which consist of 601, 635, and 628 amino acids, respectively, and have divergent C-terminal cytoplasmic domains, were isolated from rat brain by RT-PCR. Kv4.3b, d, e and f are expressed at much lower levels in brain. Mutagenesis which removed 149 amino acids in C-terminal domain of Kv4.3a significantly slowed its rate of recovery from inactivation as measured in heterologous expression in HEK293 cells. Surprisingly, however, neither the rate of inactivation nor voltage dependence of the activation and inactivation were changed.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Yuji Imaizumi et al.: "Ca^<2+> images and K^+ current during depolarization in smooth muscle cells of the quinea-pig vas deferens and urinary bladder" J.Physiol.(Lond.). 510. 705-719 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masaru Hirano et.al.: "Effects of ruthenium red on membrane ionic carrents in urinary bladder smooth muscle" Pfluger Arch.435. 645-661 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hisao Yamamura et al.: "Activation of Ca^<2+>-dependent K^+ current by nerdihydroquaiaretic acid in pocine cornary atterial smooth muscle cells" J.Pharmacol.Exp.Ther.in press. (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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