| Project/Area Number |
10672098
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
医薬分子機能学
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| Research Institution | Setsunan University |
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Principal Investigator |
KUMADAKI Itsumaro Setsunan University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (20057326)
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| Co-Investigator(Kenkyū-buntansha) |
OMOTE Masaaki Setsunan University, Faculty of Pharmaceutical Sciences, Asistant, 薬学部, 助手 (90299032)
ANDO Akira Setsunan University, Faculty of Pharmaceutical Sciences, Lecturer, 薬学部, 講師 (20138960)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Vitamin E / fluorine / liposome / ィイD119ィエD1F-NMR / relaxation time / mobility / fatty acid / phosphatidylcholine / 含フッ素脂肪酸 / フッ素NMR / ホスファチジルコン |
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| Research Abstract |
Biological activities of vitamin E are believed to be based on stabilization of biological membranes due to its anti-oxidant activity. However, some recent researches suggested that its activities are also based on mechanical stabilization of membranes. We prepared tocopherol derivatives with fluorine substituents, and studied orientation and mobility of vitamin E using relaxation time of ィイD119ィエD1F-NMR. In this research, we introduced fluorine atoms to various positions of fatty acids. Using fluorines of these acids as probes, we studied property of liposome membrane, and found that fluorines on 2- to 10-positions of stearic acid had approximately same and very short relaxation times, while those at the 12-position had much longer and at the 18-position the longest relaxation times. These results show that the outer layer of the membrane is rather rigid than the inner layer. Addition of vitamin E to these liposomes resulted in the shortening of the relaxation times. These effects were stronger in the inner part of the membrane. This result supports that vitamin E stabilizes the membrane mechanically. Syntheses of phosphatidylcholine with fluorine atoms are in progress for detailed studies.
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