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Biopharmacological study on the mechanism and the prevention of liver injury caused by organochlorine compounds which pollute the environment

Research Project

Project/Area Number 10672114
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionHoshi University

Principal Investigator

FUKUI Tetsuya  Hoshi University Pharmaceutical Sciences Professor, 薬学部, 教授 (90111971)

Co-Investigator(Kenkyū-buntansha) WATANABE Satoshi  Hoshi University Pharmaceutical Sciences Research Associate, 薬学部, 助手 (10139397)
TAKAHASHI Nonko  Hoshi University Pharmaceutical Sciences Associate Professor, 薬学部, 助教授 (50277696)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsplacenta extract / antioxidant / oxidative stress / liver injury / trichloroethylene
Research Abstract

Human placenta extract (PLx) possesses various physiologically important activities, such as antioxidant activity and anti-inflammatory activity. Therefore, purification and identification of the active antioxidants of PLx was attempted. Purification was performed successively by 40% methanol precipitation, Sephadex G-50 gel chromatography, and highperformance liquid chromatography (HPLC), and based on HPLC retention times, UV, NMR, and FAD-MS, the isolated components from PLX were identified as uracil, tyrosine, and phenylalanine. In order to confirm whether these compounds have similar function as PLx, effects of the administration of these compounds on the ethanol-induced liver injury of mice were examined. As a result, PLx suppressed ethanol-induced decrease in hepatic glutathione level, increase in thiobarbituric acid reaction substance (TBARS), increase in the activities of glutamate pyruvate transaminase, glutamate oxaloacetate transaminase, and superoxide dismutase, and decreas … More e in the activity of catalase. A mixture of uracil, tyrosine, and phenylalanine showed similar antioxidant activity to PLx, except for failure to suppress the increase in TBARS.Although these results suggest that PLx has some other unknown components to alleviate acute alcoholic liver injury, the mixture of uracil, tyrosine, and phenylalanine appeared to have almost the same ability to suppress acute ethanol-induced liver injury in mice as that of PLx.
Next, in vivo antioxidative effects of curcumin were investigated using a trichloroethylene (TCE)-induced oxidative stress model in mouse liver. Increases in the contents of peroxisome and TBARS and decreases in GSH content of mouse liver by the TCE administration were suppressed by the pre-administration of curcumin. TCE-induced changes in the activities of antioxidative enzyme, such as Cu/Zn-SOD, catalase, glutathione reductase, glutathione peroxidase (GPX) and glucose-6-phosphate dehydrogenase (G6PD), were also diminished by curcumin. These results indicate that curcumin significantly suppresses TCE-induced oxidative stress by scavenging various free radicals, and its antioxidative activity seems to be derived from its suppressive effects on the increase in peroxisome content and decrease in GPx and G6PD activities. Less

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] S.Watanabe et al.: "Effect of antioxidant on trichloroethylene-induced lives cell injury and peroxisome proliferation."Jpn.J.Toxicol.Environ.Health. 44. 4 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] J.Watanabe et al.: "Studies on the suppressive effect of antioxidant in human placental extract on oxidative stress and its mechanism."J.Health Sci.. 45. 11 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Togashi et al.: "Punification and identification of antioxidant substances in human placental extract."J.Health Sci.. 46. 117-125 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Togashi et al.: "Suppressive effects of uracil, tyrosine, and phenylalanine contained in human-placonta extract on acute ethanol-induced lives injury in mia"J.Health Sci.. 46. 126-131 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Watanabe et al.: "Suppressive effect of urcumin on frichloroethylene-induced oxidative stress."J.Nuta.Sci.Vifaminal.. 46. 230-234 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Watanabe: "Effect of antioxidant on trichloroethylene-induced liver cell injury and peroxisome proliferation"Jpn.J.Toxicol.Environ.Health. 44. 74 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Watanabe: "Studies on the suppressive effect of antioxidant in human placental extract on oxidative stress and its mechanism."J.Health Science. 45. 11 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Togashi: "Purification and identification of antioxidant substances in human-placenta extracts"J.Health Science. 46(2). 117-125 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Togashi: "Suppressive effects of Urasil, Tyrosine, and Phenylalanine contained in human-placenta extract on acute ethanolinduced liver injury in mice"J.Health Science. 46(2). 126-131 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Watanabe: "Suppressive effect of curcumin on trichloroethyleneinduced oxidative stress"J.Nutr.Sci.Vitaminol.. 46. 230-234 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Watanobe, et al.: "Effect of Antioxidant on Trichloroethylene-induced Liner Cell Injury and Peroxisome Proliferation"Jpn.J.Toxical.Environ.Health. 44. 4 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Watanabe. et al.: "Studies on the Suppressire Effect of Antioxident in Human Plauntal Extract on Oxidative Stress and its Muclanism"J.Health Sci.. 45. 11 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Togashi et al.: "Punification and identification of antioxidant substances in human-placenta extract."J.Health Sci.. 46. 117-125 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S',Togashi, et al.: "Suppressive effects of uracil, tyrosine, and phenylalanine contained in human-placenta extract on acute ethanol-induced liver injury in mice"J.Health Sci.. 46. 126-131 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Watanabe et al.: "Suppressive effect of curcumin on trichloroethylene-induced, oxidative stress."J.Nutr,Sci.Vitaminol.. 46. 230-234 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Togashi,et al.: "Punification and Identification of Antioxidant Substances in Human-Placenta Extracts"J.Health Sci. vol.46(発表予定). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Togashi,et al.: "Suppressive Effects of Unacil, Tyrosine, and Phanylalanine contained in Human-Placenta Extract on the Acute Ethanol-induced Liner Injury in Mice"J.Health Sci. vol.46(発表予定). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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