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Effect of chemokine gene transfection on acquistion of whole body tumor immunity

Research Project

Project/Area Number 10672156
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用薬理学・医療系薬学
Research InstitutionKyoritsu University of Pharmacy

Principal Investigator

NAKASHIMA Emi  Kyoritsu College of Pharmacy, Faculty of Pharmaceutical Sciences, Professor (90115254)

Co-Investigator(Kenkyū-buntansha) IIZASA Hisashi  Kyoritsu College of Pharmacy, Faculty of Pharmaceutical Sciences, Research Associate (80306662)
HATTORI Kenji  Kyoritsu College of Pharmacy, Faculty of Pharmaceutical Sciences, Research Associate (90306663)
TSUJI Akira  Kanazawa University, Faculty of Pharmaceutical Sciences, Professor (10019664)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsTUMOR IMMUNITY / BIOLOGICAL RESPONSE MODIFIER / GENE TRNSFECTION / CHEMOKINE / INDOMETHACIN / TUMOR / CYTOKINE / CONCOMITANT THERAPY
Research Abstract

The immune cells, such as T lymphocyte and the natural killer cell, act an important role in tumor regression. We studied monocyte chemotactic protein-1(MCP-1) and macrophage inflammatory protein- la(MIP-1a) those are chemotactic factor of memory T lymphocyte and the chemokine acting to monocyte which is the precursor cell of the macrophage, respectively.
We have already reported that these genes-transfected tumor cells induced apoptosis of the transplanted tumor cells. Especially, MTP- la gene-transfected tumor were rejected by the mouse of about 30%. Here, we examined the influence on the host immune response by using the Colon26clone20 colon cancer cells (hereafter, C20) introduced the gene of MCAF/MCP-1, and the synergistic administration of biological response modifier (BRM) were investigated. When both indomethacin and BRM were administered to the mouse harbored the carcinoma cell, the number of mice that rejected, and a remarkable antitumor effect was obtained. This effect was not observed in the SCID mouse that did not have T cell and B cell. Inoculated C20 cells again were also rejected. It was confirmed that the mouse obtained the tumor immunity. The antitumor effect of synergistic administration of indomethacin and BRM tended to be weakened in the mouse transplanted the MCAF/MCP-1 gene-transfected C20. For the tumor that decreases host immunity, MCAF/MCP-1 urges the macrophage permeation into the tumor, however, the activation of enough macrophage were not induced. Moreover, it was shown that the synergistic antitumor effects were obtained by using together with the medicine that adjusted the macrophage.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (17 results)

All 2000 1999 1998 Other

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (8 results) Book (1 results) Publications (5 results)

  • [Journal Article] Chemokine1999

    • Author(s)
      Nakashima E, Iizasa H
    • Journal Title

      Igakunoayumi 190(10)

      Pages: 896-901

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Journal Article] Synergistic antitumor interaction of human monocyte chemogactant protein-1 gene transfer and modulator for tumor-infiltrating macrophages.1998

    • Author(s)
      Emi Nakashima, Yuri Kubota, Ryo Matsushita, Eijiro Ozaki, Fujio Ichimura, Sakae Kawahara, Isao Nakanishi, Kouji Kuno, Kouji Matsushima
    • Journal Title

      Pharm Res 15

      Pages: 675-689

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Synergistic Antitumor Interaction of Human Monocyte Chemotactant Protein-1 Gene Transfer and Modulator for Tumor-infiltrating Macrophages1998

    • Author(s)
      Nakashima E, Kubota Y, Matsushita R, Ozaki E, Ichimura F, Kawahara S, Nakanishi I, Kuno K, Matsushima K
    • Journal Title

      Pharm Res. 15(5)

      Pages: 685-689

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Presentation] 剤形の形の意味2000

    • Author(s)
      中島恵美
    • Organizer
      第3回創薬における医薬品情報
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Presentation] Kinetic Analysis of the interaction between Valproic Acid and Meropenem in Rabbits.1999

    • Author(s)
      Matsushita R, Kubota A, Iwashita S, Ishizaki J, Yokogawa K, Nakashima E
    • Organizer
      International Conference on Drug Interaction
    • Place of Presentation
      Hamamatsu
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Presentation] 薬の上手な使い方1999

    • Author(s)
      中島恵美
    • Organizer
      第25回日本医学会総会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Presentation] 今、求められる薬剤師とは?1999

    • Author(s)
      中島恵美
    • Organizer
      第25回日本医学会総会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Presentation] Kinetic Analysis of the interaction between Valproic Acid and Meropenem Rabbits1999

    • Author(s)
      Matsushita R, Kubota A, Iwashita S, Ishizaki J, Yokogawa K, Nakashima E
    • Organizer
      International Conference on Drug Interaction
    • Place of Presentation
      Hamamatsu, in Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Presentation] Innovative usage of medicine1999

    • Author(s)
      Nakashima E
    • Organizer
      25^<th> Nihon Igakukai
    • Place of Presentation
      Tokyo, Japan(Invited lecture)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Presentation] Now, what is the requested pharmacist?1999

    • Author(s)
      Nakashima E
    • Organizer
      25^<th> Nihon Igakukai
    • Place of Presentation
      Tokyo, Japan(Invited lecture)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Presentation] Meaning of shape of dosage form

    • Author(s)
      Nakashima E
    • Organizer
      3^<rd> Souyaku niokeru iyakuhinjoho
    • Place of Presentation
      Tokyo Japan(Invited lecture)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Book] 医学の歩み1999

    • Author(s)
      中島恵美、飯笹久
    • Total Pages
      5
    • Publisher
      医歯薬出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 中島 恵美: "ケモカイン"医学のあゆみ. 190(10). 896-901 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中島 恵美: "薬物動態における遺伝子多型と相互作用"日病薬誌. 36(2). 149-152 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中島 恵美: "臨床調剤学"ミクス 東京. 13 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中島 恵美: "生物薬剤学"ミクス 東京. 19 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中島恵美: "Synergistic antitumor interaction of human monocyte chemotactant protein-1 gene transfer and modulator for tumor-infiltrating macrophages" Pharm Res. 15. 685-689 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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