| Project/Area Number |
10672171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TOHDA Shuji Tokyo Medical and Dental University, Department of Laboratory Medicine, Associate Professor, 大学院・医歯学総合研究科, 助教授 (80251510)
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| Project Period (FY) |
1998 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | acute myeloblastic leukemia / G-CSF / signal transduction |
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| Research Abstract |
I examined the responsiveness of acute myeloblastic leukemia (AML) cells for G-CSF by culturing the leukemia cells from 15 patients with or without G-CSF. I also examined the phosphorylation of intracellular proteins such as MAP kinase, JAKs, and STATs in the leukemia cells before or after the stimulation with G-CSF. I found that in the cells of which proliferation was stimulated by G-CSF, the above proteins were phosphorylated by the stimulation with G-CSF. Namely, we can infer the responsiveness for G-CSF by examining the phosphorylation much faster than in vitro culture assay. However, we could not infer whether G-CSF stimulated the self-renewal or terminal differentiation of the cells, according to the kind of phosphorylated proteins or intensity of the phosphorylation. My achievement during this term was published as shown in References.
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