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Highly sensitive analysis of the constituents in whole blood diluted with chemiluminescence-FIA using a bioreactor

Research Project

Project/Area Number 10672176
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TABATA Masayoshi  KYOTO UNIVERSITY College of Medical Technology, 医療技術短期大学部, 助教授 (70115880)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsbioreactor / chemiluminescence / whole blood glucose / FIA / pyranose oxidase / immobilized enzyme / uricase / ウリカーゼ / ピラノースオキシダーゼ / 尿酸 / フォトカウンター方式発光検出器
Research Abstract

Highly sensitive analysis of the constituents (glucose, uric acid) in whole blood diluted was tried using FIA (flow injection analysis) system involving a chemiluminescence detector (photon counting type) and an immobilized enzyme column reactor, so that a lot of constituents in blood were able to be determined with blood volume level which a mosquito drew.
1. Glucose determination-Beforehand, whole blood was diluted with physiological saline solution or distilled water, and plasma with distilled water to 5000〜400000-fold. Glucose in the diluted samples was determinated using the FIA-chemiluminescence system involving a pyranose oxidase column reactor (2x20 mm). The sample volume injected was 10 μ l. The use of a dispenser (Accu-drive, made in Jamany) and the preservation of distilled water in a glass container contributed to improve the reproducibility of glucose determination sharply. As the resuly, the reproducibility was satisfactory even the sample diluted to a hundred thousand-fold. The results of glucose concentration obtained by this method using whole blood diluted beforehand with distilled water or physiological saline solution to a hundred thousand-fold by a Accu-drive dispenser were compared with those obtained with the glucose oxidase-hydrogen peroxide electrode method. The calculated linear regression and correlation coefficient were y=0.916x-4.05 and r=0.986 for distilled water, and y=0.910x-3.00 and r=0.988 for physiological saline solution, indicating excellent correlation between the results of the two methods. In the case of plasma diluted with distilled water to a hundred thousand-fold, the calculated linear regression and correlation coefficient were y=0.979x-3.1 and r=0.987, indicating excellent agreement between the results of the two methods over a wide range of glucose concentrations.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 田畑勝好: "検査試薬としての酵素の使い方"化学と生物. 36(1). 51-56 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 田畑勝好: "バイオリアクターを中核とする希釈血液成分の高感度FIA-化学発光分析"臨床病理. 46(Suppl.). 124 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 田畑勝好: "酵素の臨床検査への応用"京臨技会誌. 26. 1-10 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masayoshi Tabata: "Highly sensitive chemiluminescence-flow injection analysis of blood glucose using a bioreactor"Proceedings of the 17th International Congress of Clinical Chemistry and Laboratory Medicine. S401 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 田畑勝好: "バイオリアクターを用いての希釈血液成分の(グルコース、尿酸)の化学発光分析"Separation Sciences 200 0講演要旨集. 37-38 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 田畑勝好: "バイオリアクターと化学発光法を用いた希釈血液成分(グルコース、尿酸)の高感度分析"臨床病理. 48(Suppl.). 3099 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 田畑勝好: "21世紀の天然・生体高分子材料 (分担執筆:診断用材料)"シーエムシー. 7 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Tabata: "How to use enzyme as examination reagents"KAGAKU TO SEIBUTSU. 36. 51-56 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Tabata: "Highly sensetive FIA-chemiluminometric analysis of blood constituents diluted with a bioreactor"Jpn.J.Clin.pathol.. 46 (Suppl.). 124 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Tabata: "Natural high molecule biomaterials in the 21th century (reagent of clinical examinations for diagnosis)"CMC. 346-352 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Tabata: "The application of enzyme to clinical examination"kyoto J.Med.Technol.. 26. 1-10 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Tabata: "Highly sensitive chemiluminescence-flow injection analysis of blood glucose using a bioreactor"Proceedings of X VII International Congress of Clinical Chemistry and Laboratory Medicine. 401 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Tabata: "Chemiluminometric analysis of blood constituents (glucose, uric acid) diluted using a bioreactor"Proceedings of Separetion Sciences 2000. 37-38 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Tabata: "Highly sensitive analysis of blood constituents (glucose, uric acid) diluted involving a bioreactor and chemiluminescence detector"Jpn.J.Clin.Pathol.. 48 (Suppl.). 3099 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 田畑勝好: "バイオリアクターを用いての希釈血液成分の(グルコース、尿酸)の化学発光分析"Separation Sciences 2000 講演要旨集. 37-38 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 田畑勝好: "バイオリアクターと化学発光法を用いた希釈血液成分(グルコース、尿酸)の高感度分析"臨床病理. 48(Suppl.). 3099 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masayoshi Tabata: "Highly sensitive chemiluminescence-flow injection analysis of blood glucose using a bioreactor"Clinical Chemistry and Laboratory Medicine. Special Suppl. S401 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田畑勝好: "酵素の臨床検査への応用"京臨技会誌. 26. 1-10 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田畑勝好: "検査試薬としての酵素の使い方" 化学と生物. 36(1). 51-56 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 田畑勝好: "バイオリアクターを中核とする希釈血液成分の高感度FIA-化学発光分析" 臨床病理. 46(Suppl.). 124 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 田畑勝好: "酵素の臨床検査への応用" 京臨技会誌. 26(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masayoshi Tabata: "Masayoshi Tabata Highly sensitive chemiluminescence-flow injection analysis of blood glucose using a bioreactor" Proceedings of the 17th International Congress of Clinical Chemistry. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 田畑勝好: "21世紀の天然・生体高分子材料(分担執筆:診断用材料)" シーエムシー, (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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