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Secretion and regulation of endothelin and ALP from human synovial cells

Research Project

Project/Area Number 10672181
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionFukushima Medical University

Principal Investigator

YOSHIDA Hiroshi  Medicine, Clin Lab., Prof., 医学部, 教授 (90045683)

Co-Investigator(Kenkyū-buntansha) IMAFUKU Yuji  Medicine, Clin Lab., associate, 医学部, 助手 (40254015)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordssynovial cells / endothelin / ALP / cytokines / 接着分子 / ヒト滑膜細胞 / A-様細胞 / A型滑膜細胞 / 慢性関節リウマチ
Research Abstract

Macrophage-like type A synoviocytes collected from RA patients were shown to secrete endothelin (ET), alkaline phosphatase (ALP) and lactic dehydrogenase.
ET-1 is secreted from macrophage-like synoviocytes, but not from fibroblast-like type B cells, RA synovial fluid cells aud 7 established human cells. Analyses by HPLC disclosed that ET-1 is the major ET in synovial fluid.(CCA 1997, ACB 1998, APCB 1998).
ALP was secreted only from type A cells, of which properties were as follows ; α-β mobility, neuraminidase sensitive, heat-stable at 56℃ for 10-30 min, slightly inhibited by L-phenylalanine and high affinity to wheat germ lectin (90%).(16th IFCC, 1998)
Morphological studies by microscopy showed that ALP was stained granular in cytoplasma and by flow cytometry CD13 was positive and CD15 and CD11b were weakly positive.
Among 24 cytokines and 5 adhesion molecules tested, MMP2, IL6. IL8 and tissue factor were detected in the cultured supernatent.(ASCP 2000, WASPAL 2001).

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Yoshida,H. et al: "Cytokine production by human synoviacytes."GCK Asian Conf Clin Pathol (Abst). 156 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshida,H. et al: "Endothelin-1 production by human synoviacytes."Ann Clin Biochem. 35. 290-294 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshida,H. et al: "Production of endothelin-1 by cultured synoviacytes."Clin Chim Acta. 259. 187-189 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Iwafuku,Y. et: "The effect of detergents on chemical occult blood test."Fukashima J Med Sci. 45. 117-123 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ozawa,Y. et: "Determination of Kflwx activity of erythrocytes"Clin Chim Acta. 279. 125-132 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshida,H. et al: "Endo.thelin concentrations in synovial fluid and sera…"Asia Pacific.Clin.Biochem. 172 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshida, H.et al.: "Cytokine production by human synoviocytes"6th ACCP. 156. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshida, H.et al.: "Endothelin-1 production by human synoviocytes."Ann.Clin.Biocheem.. 5. 290-294 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshida, H.et al.: "Production of endothelin-1 by cultured synoviocytes."Clin Chim Acta. 259. 187-189 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshida, H.et al.: "Endothelin concentrations in synovial fluids and sera of collagen diseases patients."APCB. 172. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Imafuku, Y.et al.: "The effect of detergents on chemical occult blood tests."Fukushima J Med Sci.. 45. 117-123 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ozawa, Y.et al.: "Determination of K-flux activity of erythrocytes."Clin Chim Acta. 279. 125-132 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshida,H. et al: "Cytokines secreted from human synoviocytes"Clin.Laboratory (WASPALM Dusseldorf). (発表予定). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshida,H. et al: "Cytokine production by human synoviocytes"With Asian Conf.Clin.Pathol.(Pusan). 156 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshida,Y. et: "Endothelin 1 production by human synoviocytes"Ann.Clin.Biochem. 35. 290-294 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshida,H. et: "Production of endothelin-1 by cultured synovioytes"Clin Chim Acta. 259. 187-189 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ozawa,Y. et al: "Determination of K-flux activity of erythrocytes"Clin Chim Acta. 279. 125-132 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Imafuku,Y et al: "The effect of detergents on chemical occult blood test"Fukashima J Med Sci.. 45. 117-123 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 吉田浩: "臨床検査診断マニュアル(分担)"永井書店. 6 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 吉田浩: "検査項辞苑(分担)"大塚製薬(アッセイ). 22 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshida,H.et al: "Production of endothelin-1 by cultured human synoviocytes."Clin.Chim Acta. 259. 187-189 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshida,H.et al: "Eudothelin-1 production by human synoviocytes."Ann Clin Biochem. 35. 290-294 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshida,H.et al: "Heat-stable ALP in rheumatoid arthritis pts."5th ACCP. (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ozawa,Y et al: "Determination of potassium flux activity of erythrocytes."Clin Chim Acta. 279. 125-132 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Imafuku Y.et al: "Automated measurement of ESR and its relation to ・・・・・・"Hematol Cell Ther. 40. 27-32 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshida, H. et al.: "Heat stable ALP secreted from synoviocytes of RA patients" XVI IFCC (London). (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida, H. et al.: "Endothelin-1 production by human synoviocytes" XIX WASP (Paris). (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida, H. et al.: "Production of endothelin-1 by cultured human synoviocytes" Clin Chim Acta. 259. 187-189 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida, H. et al.: "Endothelin-1 pproduction by human synoviocytes" Ann Clin Biochem. 35. 290-294 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida, H. et al.: "Heat-stable ALP in rheumatoid arthritis patients" 5th ALLP (Kohchi). (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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